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ISHzyme for ONCORE is a digestive enzyme used in the pretreatment of formalin-fixed paraffin-embedded tissues (FFPE) to enhance probe accessibility to nucleic acid targets. In FFPE tissues, certain in situ hybridization protocols require enzymatic pretreatment for proper ISH staining. ISHzyme is an aggressive enzyme and can be used at room temperature. Certain tissues require this type of aggressive enzyme digestion prior to heat retrieval for optimal results. When used in combination with ISH Retrieval for ONCORE, a synergistic effect on probe accessibility to nucleic acid targets may be achieved. This product is provided as a two-component system and may be prepared at various concentrations, as desired by the user.
1. Wilkinson DG. In Situ Hybridization: A Practical Approach (Practical Approach Series). 2nd Ed. Oxford: Oxford University Press, 1999.
2. Nuovo GJ. In Situ Molecular Pathology and Co-Expression Analyses. 1st Ed. San Diego: Academic Press, 2013.
3. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.