Phospho-Histone H3
Phospho-Histone H3 (pHH3) is a recently described immunomarker specific for cells undergoing mitosis. The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation and cell division. Histone H3 phosphorylation may initiate at different phases of cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 10 and serine 28 is coupled with mitotic chromosome segregation and condensation. Prognostic significance of the mitotic index (MI) using the Mitosis marker anti-PHH3 antibody have been reported to be of great value in breast cancer, melanoma and meningiomas. pHH3 staining may also be a useful method in which accurate determination of proliferation potential is relevant to tumor grading or clinical treatment decision-making.
| Ordering Information |
Catalog Number |
Volume |
| Concentrate |
CP 404 |
A, C |
| Predilute |
PP 404 |
AA |
| intelliPATH™ |
N/A |
N/A |
| VP Echelon |
N/A |
N/A |
| A / AK = 0.1 ml |
B / BK = 0.5 ml |
C / CK = 1.0 ml |
G5 = 5.0 ml |
AA = 6.0 ml |
H / G25 = 25 ml |
L = 100 ml |
