Wash Buffer is a ready-to-use Tris-buffered solution intended for use in washing formalin-fixed, paraffin-embedded (FFPE) tissue sections between steps of an immunohistochemistry (IHC) procedure performed on Biocare Medical’s ONCORE Pro Automated Slide Stainer. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy. Wash Buffer is used to rinse tissue sections after each incubation step, effectively removing the previous reagent and preparing the tissue for application of the following reagent. Wash Buffer is a Tris-buffered solution (pH 7.6- 7.8) with added surfactant to improve spreading and a preservative to inhibit microbial growth. Wash Buffer is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Pro Automated Slide Stainer.