Universal Negative Control Serum is a buffered solution of mouse IgG and rabbit IgG antibodies intended for use as a negative control reagent on formalin-fixed, paraffin embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure performed on Biocare Medical’s ONCORE Pro Automated Slide Stainer and visualized by light microscopy. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy. Universal Negative Control Serum should be used in place of primary antibody to confirm performance of mouse and rabbit detection systems and evaluate any staining that may occur in the absence of primary antibody. Universal Negative Control Serum is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Pro Automated Slide Stainer.