The ONCORE Pro-Tect Detection Kit is designed using a two-step or threestep method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the two-step method (rabbit), the universal linker is applied, then an additional enzyme-linked polymer labeled reagent is sequentially applied. In the three-step method (mouse), the mouse probe is applied, followed by the universal linker, then an additional enzyme-linked polymer labeled reagent is sequentially applied. The two-step and three-step methods are designed to amplify the detection in cases of low expressing antigens.
The ONCORE Pro-Tect Detection kit is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Pro Automated Slide Stainer.