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Chromogenic in situ hybridization (ISH) permits the visual identification of specific mRNA or DNA nucleic acid sequences in tissues. Following application of the probe, the presence of a target nucleic acid is visualized by the sequential application of a secondary reagent that binds the digoxigenin labeled probe, followed by a tertiary enzyme-antibody conjugate, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy.
ISH HRP Detection is comprised of a mouse anti-digoxigenin antibody as a secondary reagent and an HRP enzyme-antibody conjugate as a tertiary reagent, suitable for the detection of digoxigenin labeled probes as part of an ISH staining procedure on the ONCORE Automated Slide Stainer. ISH HRP Detection is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Automated Slide Stainer.
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2. Shibata Y, et al. Assessment of decalcifying protocols for the detection of specific RNA by non-radioactive in situ hybridization in calcified tissues. Histochem Cell Biol. 2000 Mar; 113(3):153-9.
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5. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.