Kappa and Lambda antibodies are usually run together on two separate tissues. In normal tissue, the Kappa and Lambda cell ratio is approximately 2:1. The double stain antibody allows the investigator to simultaneously see both Kappa (M) (brown) and Lambda (P) (red) on the same tissue section, thus allowing the end-user a more accurate and easier assessment of both stains.
The antibody cocktail recognizes both Kappa and Lambda light chains. It is reportedly useful in the identification of myelomas, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates may indicate that the infiltrate is malignant.