ONCORE Pro ISH HRP Detection

Product Description

Chromogenic in situ hybridization (ISH) permits the visual identification of specific mRNA or DNA nucleic acid sequences in tissues. Following application of the probe, the presence of a target nucleic acid is visualized by the sequential application of a secondary reagent that binds the digoxigenin labeled probe, followed by a tertiary enzyme-antibody conjugate, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy.

ISH HRP Detection is comprised of a mouse anti-digoxigenin antibody as a secondary reagent and an HRP enzyme-antibody conjugate as a tertiary reagent, suitable for the detection of digoxigenin labeled probes as part of an ISH staining procedure on the ONCORE Automated Slide Stainer. ISH HRP Detection is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Automated Slide Stainer.

Specifications

1. Beck RC, et al. Automated colorimetric in situ hybridization (CISH) detection of immunoglobulin (Ig) light chain mRNA expression in plasma cell (PC) dyscrasias and non-Hodgkin lymphoma. Diagn Mol Pathol. 2003 Mar; 12(1):14-20.
2. Shibata Y, et al. Assessment of decalcifying protocols for the detection of specific RNA by non-radioactive in situ hybridization in calcified tissues. Histochem Cell Biol. 2000 Mar; 113(3):153-9.
3. Wilkinson DG. In Situ Hybridization: A Practical Approach (Practical Approach Series). 2nd Ed. Oxford: Oxford University Press, 1999.
4. Nuovo GJ. In Situ Molecular Pathology and Co-Expression Analyses. 1st Ed. San Diego: Academic Press, 2013.
5. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.