Biocare’s Universal HRP Detection Kit has been developed for use with mouse and rabbit primary antibodies. This biotin-free technology uses a specific probe to detect mouse primary antibodies, which is then followed by a horseradish peroxidase (HRP) polymer that binds to the probe or to rabbit antibodies. This innovative HRP polymerization technology provides increased staining sensitivity when compared to Envision and other polymer detection kits. It is packaged for use on an automated staining system such as intelliPATH. intelliPATH Peroxidase Blocking Reagent is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations. 3, 3’ Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol and xylene. This product comes in a two- component system consisting of a liquid stable DAB chromogen and DAB substrate buffer. intelliPATH DAB is a very stable and superior formulation of DAB. In some cases, antibody titers may increase as much as two-fold. intelliPATH DAB is packaged for use on an automated staining system such as intelliPATH. intelliPATH Hematoxylin is water-based and is specially formulated for immunohistochemistry. It can be used for both paraffin and frozen sections. intelliPATH Hematoxylin stain nuclei a beautiful sky-blue and provides the end-user high contrast staining for DAB, AEC, Bajoran Purple and Fast Red or Warp Red procedures. The hematoxylin contains an anti-oxidant, and in most cases does not need filtering. It is packaged for use on an automated staining system such as intelliPATH. When used at the recommended 300uL/slide volume, approximately 260 slides can be stained using this kit. If smaller volumes are used, it will increase the total number of slides per kit.