Biocare’s Universal HRP Detection Kit has been developed for use with mouse and rabbit primary antibodies. This biotin-free technology uses a specific probe to detect mouse primary antibodies, which is then followed by a horseradish peroxidase (HRP) polymer that binds to the probe or to rabbit antibodies. This innovative HRP polymerization technology provides increased staining sensitivity when compared to Envision + and other polymer detection kits. It is packaged for use on an automated staining system such as intelliPATH. intelliPATH Peroxidase Blocking Reagent is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations. 3, 3’ Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol and xylene. This product comes in a two- component system consisting of a liquid stable DAB chromogen and DAB substrate buffer. intelliPATH DAB is a very stable and superior formulation of DAB. In some cases, antibody titers may increase as much as two-fold. intelliPATH DAB is packaged for use on an automated staining system such as intelliPATH. intelliPATH Hematoxylin is water-based and is specially formulated for immunohistochemistry. It can be used for both paraffin and frozen sections. intelliPATH Hematoxylin stain nuclei a beautiful sky-blue and provides the end-user high contrast staining for DAB, AEC, Bajoran Purple and Fast Red or Warp Red procedures. The hematoxylin contains an anti-oxidant, and in most cases does not need filtering. It is packaged for use on an automated staining system such as intelliPATH. When used at the recommended 300uL/slide volume, approximately 260 slides can be stained using this kit. If smaller volumes are used, it will increase the total number of slides per kit.