CAT Hematoxylin sections in pretreated formalin-fixed, paraffin-embedded tissue cellular preparations in is used to visualize cellular nuclei in tissue sections and immunohistochemistry.
CAT Hematoxylin is a Mayer’s Hematoxylin (Lillie modification) histological staining reagent for use in the histologic demonstration of nuclear staining. It is also used in special-staining procedures as a counterstain for nuclear demonstration. Hematoxylin solutions contain hematein and a metal mordant. The mordant provides the stain color. In the case of Mayer’s Hematoxylin (Lillie’s modification), the hematoxylin contains aluminum, giving a purple color (Nuclei after Bluing: Blue). This staining technique is used to make the distinction between a normal nucleus and an abnormal one. For histochemical purposes, hematoxylin is commonly used as a progressive stain. Slides are left in the hematoxylin solution only long enough to stain the nuclei. After staining, tissues are blued in water that has been slightly alkalinized.