Which Biocare Antibodies Are Right For Your Roche or Ventana Instrument?

With so many automated IHC instruments on the market, it is important to understand how the mechanics of your instrument can affect antibody
staining. While some instruments use cover tiles, reaction chambers, and dynamic gap technology, Roche and Ventana instrumentation utilize
a “liquid coverslip” reagent to ensure complete slide coverage and prevent tissue drying effect when combined with reaction buffer. Primary
antibody and other reagents are then dispensed into the liquid coverslip/reaction buffer combination throughout the IHC protocol. While Roche
and Ventana antibodies are optimized under these conditions, other vendors antibodies can essentially be further diluted through this process,
leading to less than optimal staining. In many cases the staining may look ‘weak’ because of the increased dilution effect of the approach.

To counteract this dilution, Biocare Medical has created a line of prediluted antibodies specially formulated for use on Roche and Ventana
platforms. This line of antibodies, called “UltraLine Antibodies”, is optimized utilizing Biocare’s ISO-certified antibody development process
on the Roche and Ventana Benchmark Ultra® slide-staining system to ensure appropriate staining intensity. To aid in optimization, protocols
utilizing ultraView or OptiView detection are specified on the corresponding antibody datasheet. The UltraLine from Biocare covers a wide range
of antibodies used in all tissue types including p63 and ERG for prostate, MSI panel for colon, and CK5/14 + p63 + CK7/18 double stain for
breast tissue.

Does this mean “normal” prediluted antibodies or concentrated antibodies will not work on Ventana instruments? Not necessarily! Alongside
UltraLine prediluted antibodies, Biocare offers protocol recommendations for a portion of their standard ready-to-use antibodies. When using
concentrated antibodies, we recommend starting test dilutions at approximately one-third to one quarter of the lowest dilution value stated on the
datasheet (i.e. if the datasheet states “1:100 to 1:200”, one should start with a 1:25 dilution).

How Biocare Antibodies Work With Liquid Coverslip Technology

 

 

 

 

 

 

 

 

 

 

 

To learn more about the Biocare’s offering for your lab, please contact us anytime at 800-799-9499 or view our full line of antibodies for your
Ventana instrument here: https://biocare.net/product-category/primary-antibodies/ventana-antibodies/.

Roche, Ventana, Benchmark Ultra, ultraView, OptiView are registered trademarks and brands under the Roche Diagnostics group and are not affiliated with Biocare Medical, LLC