Human-on-Human HRP Polymer:

A guide to improve the use of humanized therapeutic antibody screenings

Humanized antibodies are an improving and rapidly growing class of therapeutics for treatment of human disease. Their efficacy depends highly
on specificity and sensitivity for the desired target antigen. Thus, preclinical studies of potential human (and humanized) antibody therapeutics
include immunohistochemical (IHC) screening.

A major technical problem in human antibody detection lies in non-specific staining due to endogenous human IgG. To counter this problem,
Biocare created a Human-on-Human HRP-Polymer kit designed specifically for researchers utilizing humanized antibodies on human tissues for
the development of human therapeutics. This system allows for reduced and simplified protocols while rendering high sensitivity and specificity
against the target antigen of interest.

Human-on-Human HRP Polymer Technology Benefits

 

The detection kit provides reagents to tag the humanized primary antibody with a Digoxigenin (DIG) anti-Human Linker. Once labeled, the bound
primary antibody is detected by a Mouse anti-Digoxigenin secondary, followed by an anti-mouse HRP polymer. An HRP chromogen color of choice is
then applied to generate a permanently stained slide. This easy, modern method reduces non-specific staining with the added benefit of rapid screening.

 

 

 

 

 

 

 

 

 

 

 

 

To learn more about the Human-on-Human HRP Polymer, please contact us anytime at 800-799-9499 or check out the kit here:
https://biocare.net/product/human-human-hrp-polymer/.

1. Chames, P, et al. Therapeutic antibodies successes, limitations and hopes for the future. Br J Pharmacol. 2009; 157(2):220-33
2. Peuscher, A, et al. An immunohistochemical assay on human tissue using a human primary antibody. J Immunoassay Immunochem. 2014;35(3):322-34
3. Human-on-Human HRP-Polymer. (n.d.). Biocare Medical. Retrieved March 26, 2020 from https://biocare.net/product/human-human-hrp-polymer/