Carrier-Free Antibodies

BSA-free, sodium azide-free, and glycerol-free; can be customized to any combination to meet exact specifications.

Commercially available antibodies are routinely supplied in a phosphate buffered saline (PBS) solution with several carriers, such as bovine serum albumin (BSA), sodium azide, and glycerol. These additives are essential for preventing antibody contamination, preserving the antibody, and maintaining stability. For many laboratory techniques (immunohistochemistry, ELISA, Western Blot), these stabilizers do not interfere with immunodetection. However, some of these components may hinder conjugation to fluorophores, metals, and enzymes.

While BSA acts as a protein-stabilizing agent, it can reduce conjugation efficiency by competing with the primary antibody to bind to the label of interest. Sodium azide functions as a preservative but can be toxic to cells and interfere with conjugation. Therefore, in order to use these antibodies in bioconjugation techniques, some or all of these additives need to be removed from the antibody solution.

As fluorescent multiplexing and advanced mass cytometry have become increasing valuable in areas such as cancer research, translational research, and drug discovery, the need for carrier- free antibodies has risen.

Biocare Medical now offers an extensive portfolio of customizable high-quality antibodies that can be tailored to your specific needs.