NeoPATH Pro Pepsin

Product Description

The NeoPATH Pro Pepsin is designed as a digestive enzyme pretreatment of formalin-fixed, paraffin embedded (FFPE) tissues. Most immunohistochemistry (IHC) specimens are routinely fixed in formalin and, while formalin provides excellent preservation of morphology, it can greatly diminish the sensitivity to IHC. Formalin creates cross-links that alter protein biochemistry and can block antigenic sites. Antigen retrieval is needed to break these cross-links and expose the antigen’s epitope. Biocare’s Enzyme Retrieval Reagents are used in proteolytic-induced epitope retrieval (PIER) of formalin-fixed, paraffin embedded (FFPE) tissues. PIER is the process of using a solution of enzymes such as protease, trypsin or pepsin for epitope unmasking through enzymatic digestion of tissue sections. The antigen retrieval process has many variables, including the target antigen, the antibody, fixation time, and tissue processing as well as the antigen retrieval method. For the PIER method, which is the process of using a solution of enzymes for epitope unmasking through enzymatic digestion of tissue sections, the duration of fixation, incubation parameters (time, pH and temperature), and the concentration of enzymes are important factors.

Specifications