- High staining sensitivity and specificity
- Minimum cross-reactivity to endogenous IgG with proprietary blocking reagents
- Elimination of endogenous biotin
- Reduced number of IHC steps
- Use with formalin-fixed, paraffin-embedded tissues, floating sections, frozen sections and cell culture preparations
- Highly effective ancillary reagents
- Suitable for both manual and automated techniques
PromARK for Animal Tissues
What Tissues Are You Working With?
Detection & Components for Animal Tissue
Biocare’s PromARK micro-polymer detection systems are designed specifically for use on animal tissues. The micro-polymer technology provides superior sensitivity and minimum cross-reactivity to endogenous mouse and rat IgG. The Promark product line includes specially formulated heat-retrieval solutions, blocking agents, and one- and two-step detection reagents.
Free White Paper Download: Improve your IHC Detection Methods for Animal TissuesAnimal tissues contain endogenous immunoglobulins that produce significantly high levels of background staining when standard anti-mouse/anti-rabbit detection systems are employed. Biocare provides micro-polymer detection systems for use with primary antibodies from various sources (mouse, rat, rabbit, goat) on a range of tissues (mouse, rat, canine, feline, bovine, equine, porcine, ovine) which result in reduced background staining and higher sensitivity.
Rabbit-on-Rodent: Rabbit CD3 on Mouse Spleen
MM Avidin-Biotin Biotinylation Kit: Mouse PCNA + Rabbit CD3 on mouse spleen
PromARK Micro-polymer Detection Systems Reference Table
PromARK Micro-polymer | Species | Tissue Species | Label | Blocking Reagent | Retrieval Reagent |
---|---|---|---|---|---|
Mouse-on-Farma | Mouse | Bovine, Equine, Porcine & Ovine | HRP or AP | Background Punisher | Reveal / Diva / Borg |
Mouse-on-Canine | Mouse | Canine & Feline | HRP or AP | Background Punisher | Reveal / Diva / Borg |
Rabbit-on-Canine | Rabbit | Canine & Feline | HRP or AP | Background Punisher | Reveal / Diva / Borg |
Goat-on-Rodent | Goat | Mouse | HRP or AP | Rodent Block M | Rodent Decloaker |
Mouse-on-Mouse | Mouse | Mouse | HRP or AP | Rodent Block M | Rodent Decloaker |
Rabbit-on-Rodent | Rabbit | Mouse | HRP or AP | Rodent Block M | Rodent Decloaker |
Rat-on-Mouse | Rat | Mouse | HRP or AP | Rodent Block M | Rodent Decloaker |
Goat-on-Rodent | Goat | Rat | HRP or AP | Rodent Block R | Rodent Decloaker |
Mouse-on-Rat | Mouse | Rat | HRP or AP | Rodent Block R | Rodent Decloaker |
Rabbit-on-Rodent | Rabbit | Rat | HRP or AP | Rodent Block R | Rodent Decloaker |