ONCORE Pro FISH Kit contains reagents used in the pretreatment of formalin-fixed paraffin-embedded tissues (FFPE) to enhance probe accessibility to nucleic acid targets as well as a post-hybridization buffer used to eliminate non-specific binding of the probe. In FFPE tissues, FISH protocols usually require a combination of acid, heat, and enzymatic pretreatment to achieve proper staining. Hydrochloric acid (HCl, 0.2N) is a weak acid commonly used in FISH protocols. FISH Retrieval is a citrate-based heat pretreatment solution atpH 6.0. It is a non-toxic, non-flammable and odorless solution that contains sodium azide while being thimerosal-free. FISHzyme is a commonly used pepsin digestive enzyme. Combination of all 3 pretreatment reagents provides a synergistic effect on probe accessibility to nucleic acid targets. Reagent Alcohol, 70% is necessary for tissue dehydration before a probe application. PF SSC Wash Buffer is a ready-to-use saline sodium citrate buffer (pH 7.0-7.5) that contains 0.3% NP40 and Proclin 950 as a preservative.
PF SSC Wash Buffer contains 30 mM citrate, which is commonly identified as 2X SSC. This buffer is used as a post-hybridization wash buffer in FISH procedures.