ONCORE Pro CytoFISH Kit contains reagents used in the pretreatment of cytological specimens to enhance probe accessibility to nucleic acid targets as well as a post-hybridization buffer used to eliminate nonspecific binding of the probe.
Cytological specimens with FISH protocols usually require an enzymatic pretreatment to achieve proper staining. FISHzyme is a commonly used pepsin digestive enzyme. Enzyme pretreatment helps probe accessibility to nucleic acid targets. 10% Neutral Buffered Formalin diluted in PBS buffer (Fix Mix) acts as a fixative to better preserve cell morphology. CF Reagent Alcohol, 70% is necessary for tissue dehydration before a probe application. CF SSC Wash Buffer is a ready-to-use saline sodium citrate buffer (pH 7.0-7.5) that contains 0.3% NP40 and Proclin 950 as a preservative. CF SSC Wash Buffer contains 30 mM citrate, which is commonly identified as 2X SSC. Additional wash buffers FISH Wash Buffer and Hot Wash are utilized at different steps of the protocol before or after probe hybridization.