NeoPATH Pro Universal AP Polymer Kit

Product Description

The NeoPATH Pro Universal AP Polymer Kit is designed using a one-step or two-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied while in the two-step method the secondary antibody is unlabeled, and an additional enzyme-linked polymer labeled reagent is sequentially applied. The two-step method is designed to amplify the detection in cases of low expressing antigens.

Fast red is a well-established chromogen used in IHC staining protocols that in the presence of an alkaline phosphatase (AP) enzyme produces a fuchsin red precipitate that is insoluble in organic solvents and can be cover slipped with a permanent mounting media

Specifications