Ki-67 + Caspase-3 can provide information on cell death vs. cell proliferation in the same tissue section. The Ki-67 nuclear antigen is associated with cell proliferation and is used to grade proliferation rates of tumors. Ki-67 is found throughout the cell cycle that includes the G1, S, G2, and M phases; but not the G0 phase. Apoptosis has gained central importance in the study of many biological processes, including neoplasia, neurodegenerative diseases, and development. Cleaved caspase-3 detects endogenous levels of the large fragment of activated caspase-3, a protease that mediates apoptosis. Caspase-3 does not cross react with other cleaved caspases.
Datasheets & SDS
1. Gown AM, Willingham MC. J Histochem Cytochem. 2002 Apr; 50(4):449-54.
2. Bouzubar N, et al. Br J Cancer. 1989 June; 59(6):943-7.
3. Brown RW, et al. Clin Cancer Res. 1996 Mar; 2(3):585-92.
4. Veronese SM, et al. Cancer. 1993 Jun; 71(12):3926-31.
5. Wang L, et al. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Mar; 33(3):222-6.
6. Chrysomali E, et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Nov; 96(5):566-72.