Rabbit HRP Detection is a horseradish peroxidase (HRP)-antibody conjugate system intended for use in the detection of rabbit IgG primary antibodies on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure performed on Biocare Medical’s ONCORE Pro Automated Slide Stainer and visualized by light microscopy. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy. Rabbit HRP Detection is an HRP enzyme-antibody conjugate suitable for the detection of rabbit IgG primary antibodies as part of an IHC staining procedure on the ONCORE Pro Automated Slide Stainer. Rabbit HRP Detection is prepared using a proprietary micropolymer technology that provides a highly sensitive detection system, with minimal background staining. Rabbit HRP Detection does not use biotin or streptavidin, thus avoiding non-specific staining associated with endogenous biotin. Rabbit HRP Detection is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Pro Automated Slide Stainer.