The emergence of new strains of resistant mycobacterium tuberculosis has created interest in clinical diagnosis. Studies have shown immunohistochemical and immunofluorescent techniques to be superior to conventional special stains in the detection of mycobacterium. Demonstrating mycobacterial antigens is useful in establishing mycobacterial etiology and can be used as an alternative method to the conventional Ziehl-Neelsen method. Studies have shown that Mycobacterium tuberculosis antibody is reactive with other mycobacteria species, but is not reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitides.
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