This digoxigenin-labeled oligonucleotide probe recognizes human 28S ribosomal RNA within tissue sections (1,2). This probe can be used as a control when running specific RNA targeting probes. It should be used to assess RNA integrity in FFPE tissue sections. Weak or light staining in a test sample indicates that specifically targeted mRNA may be compromised.
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2. Paillasson S, et al. In situ hybridization in living cells: detection of RNA molecules. Exp Cell Res. 1997 25:231(1):226-33.
3. Wilkinson DG. In Situ Hybridization, A Practical Approach, Oxford University Press (1992) ISBN 0 19 963327 4.
4. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
5. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.