Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy.
Mouse Amp HRP Detection is an HRP enzyme-antibody conjugate suitable for the detection of mouse IgG and mouse IgM primary antibodies as part of an IHC staining procedure on the ONCORE Automated Slide Stainer. Mouse Amp HRP Detection is prepared using a proprietary micropolymer technology that provides a highly sensitive detection system, with minimal background staining. Mouse Amp HRP Detection does not use biotin or streptavidin, thus avoiding non-specific staining associated with endogenous biotin.
Mouse Amp HRP Detection is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Automated Slide Stainer. Mouse Amp HRP Detection is recommended for use with specific primary antibodies, as indicated in the individual antibody data sheets.