Dewax Solution 3 (DS3) and Dewax Solution 4 (DS4) are water-based solutions for the removal of paraffin wax from FFPE tissue specimens, as part of an in situ hybridization staining procedure on the ONCORE Pro Automated Slide Stainer. Removal of paraffin wax must be performed prior to probe hybridization and the application of reagents necessary […]
This digoxigenin-labeled oligonucleotide probe recognizes human 28S ribosomal RNA within tissue sections (1,2). This probe can be used as a control when running specific RNA targeting probes. It should be used to assess RNA integrity in FFPE tissue sections. Weak or light staining in a test sample indicates that specifically targeted mRNA may be compromised.
Chromogenic in situ hybridization (ISH) permits the visual identification of specific mRNA or DNA nucleic acid sequences in tissues. Following application of the probe, the presence of a target nucleic acid is visualized by the sequential application of a secondary reagent that binds the digoxigenin labeled probe, followed by a tertiary enzyme-antibody conjugate, and a […]
This RNA negative control probe consists of a random set of oligonucleotide sequences with a GC content of 40-70%. It may aid in assessing non-specific staining when performing in situ hybridization in FFPE issues. No positive staining should result with the use of this probe
Immunoglobulin kappa light chain mRNA may be detected in normal and neoplastic B-cells in human lymphoid tissue (1-3). Kappa Light Chain Probe is a digoxigenin-conjugated DNA oligonucleotide designed to bind human kappa light chain mRNA.
Gene rearrangement in t(14;18)/IGH-MALT1 was detected in MALT lymphoma first. The frequencies at which the translocation occurs vary markedly with the primary site of the disease1. IGH-MALT1 rearrangements were described also in other B-NHLs such as DLBCL (Diffuse Large B-Cell Lymphoma)2. IGH-MALT1 fusion in the t(14;18)(q32;q21) have been identified in marginal zone B-cell lymphomas of […]
The Copy Control Copy Control 20q11.2 Aqua FISH Probe is a fluorescently labeled probe designed to hybridize to ~739kb of the 20q11.2 region of chromosome 20. Copy Control 20q11.2 Aqua probe (20 ng/µl) is provided in FISH Hybridization Buffer (FRR7311A) containing dextran sulfate and formamide.
The ALK cen (2p23.2) Green FISH Probe is a fluorescently labeled probe designed to hybridize to ~ 498 kb of the 2p23.2 region of chromosome 2. ALK cen (2p23.2) Green probe (120 ng/µl) is provided in FISH Hybridization Buffer (FRR7311A) containing dextran sulfate and formamide.
The ALK tel (2p23.2) Orange FISH Probe is a fluorescently labeled probe designed to hybridize to ~ 490 kb of the 2p23.2 region of chromosome 2. ALK tel (2p23.2) Orange probe (60 ng/µl) is provided in FISH Hybridization Buffer (FRR7311A) containing dextran sulfate and formamide.
Loss of the 13q region or even the whole of chromosome 13 is very common in cases of multiple myeloma (MM). Deletions of the RB1 gene are common in a variety of hematological malignancies including chronic lymphocytic leukemia (CLL), acute myelocytic leukemia (AML) and MM1,2,3. FOR DISTRIBUTION OUTSIDE THE UNITED STATES ONLY
