This digoxigenin-labeled oligonucleotide probe recognizes human 28S ribosomal RNA within tissue sections (1,2). This probe can be used as a control when running specific RNA targeting probes. It should be used to assess RNA integrity in FFPE tissue sections. Weak or light staining in a test sample indicates that specifically targeted mRNA may be compromised.
Immunoglobulin kappa light chain mRNA may be detected in normal and neoplastic B-cells in human lymphoid tissue (1-3). Kappa Light Chain Probe is a digoxigenin-conjugated DNA oligonucleotide designed to bind human kappa light chain mRNA.
CD137, a member of the tumor necrosis factor receptor superfamily, represents a promising target for enhancing antitumor immune responses (1). The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis (2). CD137 helps regulate the activation of many immune cells, including CD4(+) T cells, CD8(+) T cells, dendritic cells, and natural killer […]
The Ki-67 nuclear antigen is associated with cell proliferation and found throughout the cell cycle; though not in G0 phase (1,2). The assessment of Ki-67 proliferation in breast cancers has shown the Ki-67 labelling index is an important predictor of survival (3). Cytokeratin (CK) 7 is expressed in epithelial cells of the ovary, lung and […]
IMP3 (insulin-like growth factor II mRNA-binding protein 3) is a 580 amino acid oncofetal RNA binding protein containing four K homology domains which is encoded by a 4350 bp mRNA transcript produced by the IGF2BP3 gene on chromosome 7p11.5 (1,2). Its relevance as a novel biomarker in many kinds of cancer has been recently published […]
The Ki-67 nuclear antigen is associated with cell proliferation and found throughout the cell cycle; though not in G0 phase (1,2). The assessment of Ki-67 proliferation in breast cancers has shown the Ki-67 labelling index is an important predictor of survival (3). Microscopic evaluation of mitotic figures on H&E is a routine procedure in the […]
A monoclonal antibody cocktail targeted against mini-chromosome maintenance protein 2 (MCM2) and DNA topoisomerase IIA (TOP2A), when up regulated, serves as a marker of aberrant S-phase induction in proliferating cells.15 MCM2, as part of a hexameric pre-replication complex, drives the formation of pre-replicative complexes during DNA replication, a critical initial step during G1 phase.16 TOP2A […]
The monoclonal antibody against HBME-1 targets an unknown antigen of mesothelial microvilli (1). HBME-1 is abnormally expressed in thyroid cancer, showing cytoplasmic location with membrane accentuation (2-5). Anti-HBME-1 has been most extensively investigated as an immunochemical (IHC) marker in thyroid cancer. It has a sensitivity of 78.3% and specificity of 85.4% in 10 studies using […]
CD1a is a protein of 43 to 49 kDa and is expressed on dendritic cells and cortical thymocytes (1,2). CD1a [O10] staining has been shown to be useful in the differentiation of Langerhans cells from interdigitating cells. It has also proved useful for phenotyping Langerhans cell histiocytosis (2,3). CD1a may be a novel biomarker for […]
CD4 is a transmembrane glycoprotein, expressed on normal thymocytes, T-helper cells, the majority of mature peripheral T cells, and a subset of suppressor or cytotoxic T cells (1). Like many cell surface receptors/markers, CD4 is a member of the immunoglobulin superfamily. CD4 is expressed in the majority of T-cell lymphomas, including mycosis fungoides (2). CD4 […]
