MART-1 recognizes an 18 kDa protein (Melanoma Antigen Recognized by T cells 1) and is specific for melanocytic lesions, making it a valuable component of melanoma panels.15-17 MART-1 has been shown to be more sensitive than HMB45 for labeling metastatic melanomas. Tyrosinase, a key enzyme in early melanin biosynthesis, is more sensitive than HMB45 and […]
Melan-A (MART-1) [A103], a melanoma-specific antigen, is a transmembrane protein and a melanocyte differentiation marker recognized by cytotoxic T lymphocytes. Melan-A is expressed in skin, in the majority of melanocytes and in renal angiomyolipomas.15-17
Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase antibody to be a more sensitive marker for melanoma when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45. Unlike HMB45, Tyrosinase does not discriminate between activated or […]
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. The S100 Protein Cocktail antibody stains melanocytes, schwannomas, peripheral neural tissue, astrocytes, benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhan’s cells in skin and interdigitating reticulum cells […]
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. The S100 antibody stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhan’s cells in skin and interdigitating reticulum cells in the paracortex […]
MART-1 recognizes a protein of 18 kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells).1 MART-1 is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions.1,2 Studies have shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas.3 This MART-1 cocktail does not stain steroid tumors. Tyrosinase […]
Nerve Growth Factor Receptor antibody (NGFR) is a useful immunohistochemical marker, when used in conjunction with the S100 antibody, to aid in the diagnosis of desmoplastic and neurotrophic malignant melanomas, which are often negative for other melanocytic markers (HMB45, MART-1/Melan-A). Studies have shown that NGFR is expressed on Schwann cells, neuronal axons and perineural cells, […]
Microphthalmia Transcription Factor antibody (MiTF) is a nuclear melanocytic marker. Studies have shown it is a sensitive and specific marker for malignant melanoma, including some spindle-cell variants. MiTF has been shown to have superior sensitivity and specificity to S100 and HMB45. MiTF may be useful for identification of melanoma, melanocytic soft tissue tumors and the […]
The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells. It is also thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. The […]
