The NeoPATH Pro Antigen Retrieval 1 (AR1) is designed as a Tris-EDTA-based buffer used in heat-induced antigen retrieval (HIER) of formalin-fixed, paraffin-embedded (FFPE) tissues. HIER is the process of heating the slide-mounted specimen material in an antigen retrieval solution, followed by a cooling-off period. Retrieval solutions with paraffin emulsifiers (surfactants) followed by a rinsing reagent […]
The NeoPATH Pro Universal AP Plus Polymer Kit is designed using a one-step or two-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied while in the two-step […]
The NeoPATH Pro Rabbit Amp AP Plus Kit is designed using a one-step method for detecting rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micropolymer is applied and an additional enzyme-linked polymer labeled reagent is […]
Transducin-like enhancer of split 1 (TLE1) is a member of the TLE family of transcriptional co-repressors that control the transcription of a wide range of genes and has been implicated in embryogenesis, hematopoiesis, and neuronal and terminal epithelial differentiation.15 TLE1 is involved in the Wnt/β-catenin signaling pathway, which is important in synovial sarcomas, and in […]
The NeoPATH Pro Multiplex Detection 2 Kit is designed using a one-step method for detecting mouse and rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied. NeoPATH Pro Multiplex Detection 2 Kit is […]
The NeoPATH Pro Rabbit Amp AP Kit is designed using a one-step method for detecting rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied and an additional enzyme-linked polymer labeled reagent is sequentially […]
The NeoPATH Pro-Tect HRP Detection Kit is designed using a two-step or three-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the two-step method (rabbit), the universal linker is applied, then an additional enzyme-linked polymer labeled reagent is sequentially applied. […]
The NeoPATH Pro Universal AP Polymer Kit is designed using a one-step or two-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied while in the two-step method […]
The Probe Cleaning Kit includes reagents that clean the tube circuits by removing DAB residual and wax residues in the NeoPATH Pro Inmunostainer. The kit will indicate the schedule and the procedure to correctly perform this type of cleaning to avoid interference with the performance of the IHC protocol.
TBS Tween Buffer 20 10X is a buffered saline solution used as a washing reagent in immunohistochemical and in situ hybridization procedures. Tween 20 acts by permeabilizing cell membranes and favoring antibodies penetration in the tissue section facilitating the specific binding. In any immunohistochemical procedure, any antibody that is not bound to the antigen during […]
