MOC-31, also known as Epithelial Specific Antigen/Ep-CAM, consists of two 34 and 39 kDa glycoproteins. These glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells.15-18
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The Breakdown: Enzymes and Epitope Retrieval Tissue fixation can lead to protein cross-linking, which can block antigenic sites and limit antigen-antibody binding. To break these cross links and expose the antigen’s epitope, antigen retrieval will be necessary. The two most common methods to recover masked epitopes are heat induced epitope retrieval (HIER) and proteolytic antigen […]
