Heat-induced epitope retrieval methods (HIER) are used to pretreat tissues for optimum antibody-antigen reaction. Many different kinds of heat retrieval methods are used. These include the following: microwave, waterbath, steamers and pressure cooker methods. These methods were developed to “Standardize” immunohistochemistry for formalin-fixed, paraffin-embedded tissues. Heat retrieval methods’ lack of “standardization” and quality control (QC) […]
The NeoPATH Pro Universal AP Plus Polymer Kit is designed using a one-step or two-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied while in the two-step […]
The NeoPATH Pro Antigen Retrieval 1 (AR1) is designed as a Tris-EDTA-based buffer used in heat-induced antigen retrieval (HIER) of formalin-fixed, paraffin-embedded (FFPE) tissues. HIER is the process of heating the slide-mounted specimen material in an antigen retrieval solution, followed by a cooling-off period. Retrieval solutions with paraffin emulsifiers (surfactants) followed by a rinsing reagent […]
The NeoPATH Pro Rabbit Amp AP Plus Kit is designed using a one-step method for detecting rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micropolymer is applied and an additional enzyme-linked polymer labeled reagent is […]
Transducin-like enhancer of split 1 (TLE1) is a member of the TLE family of transcriptional co-repressors that control the transcription of a wide range of genes and has been implicated in embryogenesis, hematopoiesis, and neuronal and terminal epithelial differentiation.15 TLE1 is involved in the Wnt/β-catenin signaling pathway, which is important in synovial sarcomas, and in […]
The NeoPATH Pro Multiplex Detection 2 Kit is designed using a one-step method for detecting mouse and rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied. NeoPATH Pro Multiplex Detection 2 Kit is […]
The NeoPATH Pro Rabbit Amp AP Kit is designed using a one-step method for detecting rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied and an additional enzyme-linked polymer labeled reagent is sequentially […]
The NeoPATH Pro-Tect HRP Detection Kit is designed using a two-step or three-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the two-step method (rabbit), the universal linker is applied, then an additional enzyme-linked polymer labeled reagent is sequentially applied. […]
The NeoPATH Pro Universal AP Polymer Kit is designed using a one-step or two-step method for detecting mouse and/or rabbit primary antibodies to form an antibody-enzyme complex. This complex is then visualized using an appropriate substrate/chromogen. In the one-step method a secondary antibody directly linked to the micro-polymer is applied while in the two-step method […]
The ready-to-use Low-AR solution is used for heat-induced antigen retrieval as part of an IHC staining procedure in the NeoPATH Pro automated stainer.
