TBS Tween Buffer 20 10X is a buffered saline solution used as a washing reagent in immunohistochemical and in situ hybridization procedures. Tween 20 acts by permeabilizing cell membranes and favoring antibodies penetration in the tissue section facilitating the specific binding. In any immunohistochemical procedure, any antibody that is not bound to the antigen during […]
![Tonsil stained with CD20 [BC30] antibody](https://biocare.net/wp-content/uploads/3287.jpg)
CD20 is a B-lymphocyte antigen, composed of an activated-glycosylated phosphoprotein which is expressed on the surface of all B-cells.15 CD20 is involved in the regulation of trans-membrane Ca2+ conductance and plays critical roles in cell‐cycle progression during human B cell proliferation and activation.15 CD20 is expressed on more than 90% of B-cell lymphoma cells making […]
CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), can hydrolyze extracellular ATP and ADP. It is one of the key enzymes in the adenosine pathway and targeted adenosine may play a role in antitumor strategies.15 CD39 pathway inhibits natural killer (NK) cells and promotes the metastasis of tumors.15 Overexpression of CD39 has been observed […]
![Melanoma stained with PRAME [EPR20330] antibody](https://biocare.net/wp-content/uploads/3252.jpg)
PRAME is located on chromosome 22q11.22 and encodes a 509 amino acid protein. PRAME is an autosomal cancer-testis antigen (CTA) gene which has been shown to be expressed in melanoma, various nonmelanocytic malignant neoplasms, including non-small cell lung cancer, breast carcinoma, renal cell carcinoma, ovarian carcinoma, leukemia, synovial sarcoma, and myxoid liposarcoma. Normal healthy tissues […]
E-Cadherin is a transmembrane glycoprotein that plays a key role in cellcell adhesion in epithelial tissues (1-2). The adherens junction between epithelial cells is comprised of extracellular domains of E-Cadherin from adjacent cells, which interact through a molecular zipper motif. In normal tissues, immunostaining of E-Cadherin is localized to the membrane of epithelial cells, consistent […]
Programmed death 1 (PD-1) is a cell surface co-receptor member of the CD28/CTLA-4 family, and functions as a downregulator of the immune system through a dual mechanism of inhibition (1). PD-1 is expressed on the cell surface of activated T- and B-cells. Anti-tumor immunity may be controlled by the PD-1/PD-L1 signaling pathway. PD-L1, one of […]
Val Wash Buffer, 20X (sodium azide and thimerosal free) is a high quality TBS buffer with superior pH stability. The buffer contains a surfactant that allows reagents to spread uniformly across tissue sections on slides. It is designed for use on the VALENT Automated Slide Staining Platform.
Val Aqua Rinse, 20X contains a nonionic polysorbate detergent (surfactant) commonly used as an additive to buffers and reagents in IHC procedures to enhance spreading across tissues and reduce background staining. Val Aqua Rinse, 20X has been designed to be diluted in deionized water for rinsing specimens on the VALENT Automated Slide Staining Platform.
The Copy Control Copy Control 20q11.2 Aqua FISH Probe is a fluorescently labeled probe designed to hybridize to ~739kb of the 20q11.2 region of chromosome 20. Copy Control 20q11.2 Aqua probe (20 ng/µl) is provided in FISH Hybridization Buffer (FRR7311A) containing dextran sulfate and formamide.
Cervical cancer is one of the most common cancers affecting women worldwide. The implementation of cytogenetic based screening techniques has greatly reduced the incidence and mortality of cervical cancer in the United States 1. Moreover, traditional screening methods such as Pap smears and human papillomavirus (HPV) based testing are utilized to stratify and identify patients […]
