Decloaking Chamber™ NxGen
The Decloaking Chamber™ NxGen has been designed for heat-induced epitope retrieval (HIER) and ease of use. It has 5 discrete temperature settings ranging between 6 0ºC and 110 ºC with user programmable times. The 110 ºC antigen retrieval protocol can be completed from start to finish in under an hour. With a 72 total slide capacity and only minutes of hands-on time per run, the Decloaking Chamber NxGen offers a walk-away capability similar to fully automated staining instruments.
The Decloaking Chamber NxGen transfers run data to a USB drive for export to a user’s computer. The run data recorded includes the date and time per run with temperature and pressure readings throughout. With the Decloaking Chamber NxGen recalling the settings from the last run, a quick start of the same protocol is possible.
The Decloaking Chamber NxGen is an excellent tool for HIER. The proper use of heat and pressure in conjunction with the appropriate buffer solutions is of the utmost importance for consistent immunohistochemistry (IHC) staining. The Decloaking Chamber NxGen is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety and quality control requirements. Temperature, pressure and time can be monitored and recorded with the Decloaking Chamber NxGen to produce consistent staining.
CE Listed, ETL approved, U.S. patent number 6,580,056, June 17, 2003
- Crisp and even staining
- Very gentle on tissues
- Reproducible results
- Reduction of false negatives
Quality Controlled System
- pH indicators change color with temperature
- Two-step deparaffinization and retrieval buffers
- Steam monitor strips for temperature verification
- Audible alert upon run completion
|Decloaking Chamber||Description||Cat. No.||Volume|
|Decloaking Chamber™ NxGen||(For use in 110 V markets)||DC2012||1 ea.|
|Decloaking Chamber™ NxGen||(For use in 220 V markets)||DC2012-220V||1 ea.|
For pricing and availability of our instrumentation, please contact us.
|Size||14.2” x 13.5” x 13” / 36.1 cm x 34.3 cm x 33.0 cm|
|Weight||13 lbs / 6.91 kg|
|Temperature Range||60 ºC – 110 ºC (+/- 5 ºC)|
|Slide capacity||72 total slides (3 slide canisters of 24 slides each)|
|Power Requirements||115V/60Hz/1000W or 230V/50Hz/1000W|
Decloaking Chamber™ Plus (Discontinued)
The Decloaking Chamber™ Plus offers a combination of complete walk-away and quality control capabilities, ensuring optimal antigen retrieval with superior and consistent results. This system features a cooling fan for more rapid antigen retrieval, producing results in as little as 35 minutes. An optional stainless steel rack holder allows easy removal of plastic slide containers. Furthermore, a USB port enables the laboratory to utilize a flash drive to track and save run data for quality control purposes. The pressure gauge has a new feature in which the needle remains at the highest pressure reading during the run, validating that antigen retrieval has occurred properly. When the procedure is complete, an audible beep alerts the user. The Decloaking Chamber™ Plus, with up to 96-slide capacity, is well suited for high-throughput antigen retrieval.
|Size||12″ (W) x 11″ (D) x 11″ (H) / 31cm (W) x 29cm (D) x 28cm (H)|
|Weight||8.2 lbs, 3.7 kg|
|Temperature Range||37°C to 125°C (± 5°C)|
|Pan Capacity||3.2 liters (maximum)|
|Slide capacity||Up to 96 slides (in plastic Tissue Tek containers)
Up to 60 slides (in plastic Coplin jars)
Decloaking Chamber™ Pro (Discontinued)
A Digital Programmable Pressure System for HIER. Specifically for heat-induced epitope retrieval, the Decloaking Chamber Pro is an excellent tool for optimized, consistent immunohistochemistry staining, in conjunction with the appropriate buffer solution.
|Size||13” (W) x 12” (D) x 12.5” (H) / 33 cm (W) x 30 cm (D) x 32 cm (H)|
|Weight||10.5 lbs. 4.8 kg|
|Temperature Range||37 °C to 125 °C (+/- 5 °C)|
|Pan Capacity||3.2 liters (maximum)|
|Basket, Rack Holder|
|Metal Slide Canister|
|Kling-On Microscope Slides|
|Sealing Gasket Kit|
|Pressure Limit Valve|
|Flash Drive with Software & Manual|
Ancillaries for Decloaking Chamber 2002/08
|Kling-On Microscope Slides|
|Tissue Tek Slide Holders and Containers|
Steam Monitor Strips for Quality Control for all Decloaking Chamber Models
The steam monitor strips are heat and pressure sensitive, acting as a quality control check and confirms that the chamber has reached full temperature and pressure as evidenced by the dark brown to black color. The strips can be placed in your laboratory notebook as a permanent record for that run.
pH Strips for Quality Control for all Decloaking Chamber Models
The pH of citrate-based retrieval solutions increase as the temperature rises. To assure quality control and consistent IHC staining, Biocare has developed heat-tested pH strips that give accurate and the true pH at high temperatures. The pH of a solution should be recorded at the temperature used. Using these pH strips, the laboratory investigator can now record pH on a daily basis, and comply with CAP and JCOH inspection.
|Steam Monitor Strips|
|QC pH Strips pH 4-7|
|QC pH Strips pH 7.5-10.5|
Microtiter Plate Platform™ for Decloaking Chamber Plus
The Microtiter Plate Platform facilitates antigen retrieval for free floating sections in preparation for IHC or IF and is particularly useful for whole brain sections. The platform holds microtiter plates containing tissues away from the source of heat during antigen retrieval in an HIER device. This allows for more even, standardized results for immunohistochemistry and immunofluorescence (IF) staining techniques.
Standard Microtiter Plates holding free-floating sections are filled with a retrieval solution, placed on the Microtiter Plate Platform, and lowered into the Decloaking Chamber Pro. This is especially critical when using thick sections of delicate tissue such as brain tissues. The Microtiter Plate Platform also stabilizes the specimens and enables ease of use. It has been rigorously tested for heat stability and is ISO compliant.
|Microtiter Plate Platform|
This quick and informative video series shows how easy it is to unpack and operate the Decloaking Chamber NxGen.
Decloaking Chamber NxGen New Installation
With only a few minutes of unpacking, the DC NxGen will be ready to perform heat induced epitope retrieval (HIER) on microscope slides. This video shows what is in the package and how to unpack the unit.
Decloaking Chamber NxGen Set-up and Loading
See how easy it is to operate the Decloaking Chamber NxGen. The video below demonstrates the quick set-up and proper loading of the Decloaking Chamber NxGen in order to perform heat induced epitope retrieval (HIER) on microscope slides.
Decloaking Chamber NxGen Run Reporting
The Decloaking Chamber NxGen comes with advanced easy-to-use run reporting software. See how to use the software and what reports you can create from the Decloaking Chamber NxGen data.