PromARK

Detection & Components for Animal Tissue

Biocare’s PromARK micro-polymer detection systems are designed specifically for use on animal tissues. The micro-polymer technology provides superior sensitivity and minimum cross-reactivity to endogenous mouse and rat IgG. The Promark product line includes specially formulated heat-retrieval solutions, blocking agents, and one- and two-step detection reagents.

  • High staining sensitivity and specificity
  • Minimum cross-reactivity to endogenous IgG with proprietary blocking reagents
  • Elimination of endogenous biotin
  • Reduced number of IHC steps
  • Use with formalin-fixed, paraffin-embedded tissues, floating sections, frozen sections and cell culture preparations
  • Highly effective ancillary reagents
  • Suitable for both manual and automated techniques

Animal tissues contain endogenous immunoglobulins that produce significantly high levels of background staining when standard anti-mouse/anti-rabbit detection systems are employed. Biocare provides micro-polymer detection systems for use with primary antibodies from various sources (mouse, rat, rabbit, goat) on a range of tissues (mouse, rat, canine, feline, bovine, equine, porcine, ovine) which result in reduced background staining and higher sensitivity.



Rabbit-on-Rodent: Rabbit CD3 on Mouse Spleen

Rabbit-on-Rodent: Rabbit CD3 on Mouse Spleen


Mouse-on-Mouse Avidin-Biotin Biotinylation Double Stain Kit: Mouse PCNA + Rabbit CD3 on mouse spleen

MM Avidin-Biotin Biotinylation Kit: Mouse PCNA + Rabbit CD3 on mouse spleen


PromARK Micro-polymer Detection Systems Reference Table

PromARK Micro-polymer Species Tissue Species Label Blocking Reagent Retrieval Reagent
Mouse-on-Farma Mouse Bovine, Equine, Porcine & Ovine HRP or AP Background Punisher Reveal / Diva / Borg
Rabbit-on-Farma Rabbit Bovine, Equine, Porcine & Ovine HRP or AP Background Punisher Reveal / Diva / Borg
Mouse-on-Canine Mouse Canine & Feline HRP or AP Background Punisher Reveal / Diva / Borg
Rabbit-on-Canine Rabbit Canine & Feline HRP or AP Background Punisher Reveal / Diva / Borg
Goat-on-Rodent Goat Mouse HRP or AP Rodent Block M Rodent Decloaker
Mouse-on-Mouse Mouse Mouse HRP or AP Rodent Block M Rodent Decloaker
Rabbit-on-Rodent Rabbit Mouse HRP or AP Rodent Block M Rodent Decloaker
Rat-on-Mouse Rat Mouse HRP or AP Rodent Block M Rodent Decloaker
Goat-on-Rodent Goat Rat HRP or AP Rodent Block R Rodent Decloaker
Mouse-on-Rat Mouse Rat HRP or AP Rodent Block R Rodent Decloaker
Rabbit-on-Rodent Rabbit Rat HRP or AP Rodent Block R Rodent Decloaker

Rodent Tissues

Mouse Primary Antibodies



Mouse-on-Mouse

This novel detection technology allows for use of mouse primary antibodies on mouse tissues. Available as a bundle consisting of detection micro-polymer, blocking and HIER solution or available individual components.

Mouse-on-Mouse: Mouse Hepatocyte Specific Antigen on mouse liverMouse-on-Mouse: Mouse Hepatocyte Specific Antigen on mouse liver


Mouse-on-Rat

Use with mouse primary antibodies on rat tissues. Available as a bundle containing detection polymer, blocking and HIER solution or available individually.

Mouse-on-Rat: Mouse Neurofilament on rat brainMouse-on-Rat: Mouse Neurofilament on rat brain


Ordering Information

Mouse-on-Mouse HRP-Polymer Bundle

$278.00$1,416.00 Select options

Mouse-on-Mouse HRP-Polymer

$251.00$9,466.00 Select options

Mouse-on-Mouse AP-Polymer

$267.00$741.00 Select options

Mouse-on-Rat HRP-Polymer Bundle

$243.00$833.00 Select options

Mouse-on-Rat HRP-Polymer

$212.00$1,878.00 Select options

Mouse-on-Rat AP-Polymer

$226.00$759.00 Select options

Rat Detection Kit for Anti-Mouse CD31

$246.00 Add to cart

Rat Primary Antibodies

Rat-on-Mouse Micro-polymer Detection

Use with rat monoclonal antibodies on mouse tissues. The Rat-on-Mouse HRP / AP micro-polymer detection is a two-step system. It is comprised of two reagents: a rat probe that binds to the rat primary antibody; and an HRP / AP micro-polymer that conjugates to the probe.

  • 20 – 40 times more sensitive than conventional conjugated mouse adsorbed anti-rat HRP-secondary detection
  • Use rat primary antibodies on mouse tissues
  • mouse adsorbed anti-rat detection systems display minimum cross-reactivity to endogenous mouse IgG

Ordering Information

Rat-on-Mouse HRP-Polymer

$246.00 Add to cart

Rat HRP-Polymer, 1-Step

$99.00$886.00 Select options

Rat-on-Mouse AP-Polymer

$151.00$407.00 Select options

Rabbit Primary Antibodies

Rabbit-on-Rodent Micro-polymer Detection

Rabbit-on-Rodent detection is available as a micro-polymer only.

Ordering Information

Rabbit-on-Rodent HRP-Polymer

$194.00$1,496.00 Select options

Rabbit-on-Rodent AP-Polymer

$212.00$617.00 Select options

Goat-on-Rodent Primary Antibodies

Goat-on-Rodent Micro-polymer Detection

Use with goat primary antibodies on mouse, rat and human tissues. The goat detection is a two-step system.

  • 25 – 50 times more sensitive than conventional mouse anti-goat HRP secondary detection systems
  • 10 – 20 times more sensitive than conventional mouse anti-goat AP secondary detection systems
  • Goat secondary detections display minimum cross-reactivity to endogenous mouse & rat IgG



Goat anti-E-cadherin on mouse liver (DAB)

Goat anti-E-cadherin on mouse liver (DAB)


Goat anti-E-cadherin on mouse liver (FR)

Goat anti-E-cadherin on mouse liver (FR)


Ordering Information

Goat-on-Rodent HRP-Polymer

$150.00$1,197.00 Select options

Goat-on-Rodent AP-Polymer

$154.00$415.00 Select options

Retrieval and Blocking Reagents

Blocking & HIER reagents to enhance specificity & reduce / eliminate background

Rodent Decloaker

This antigen retrieval buffer is specially formulated to work with Biocare’s Promark detection systems. Rodent Decloaker with proprietary blocking reagents help reduce mouse or rat endogenous IgG in rodent tissue. This is particularly useful for brain tissue; helps reduce nonspecific background staining and performs antigen retrieval. Antibody titers can be doubled or tripled when using Rodent Decloaker compared to the use of citrate buffer.

Rodent Block R

This blocking reagent, designed for use exclusively with rat tissue, makes use of proprietary blocking agents to reduce and/or eliminate endogenous rat IgG. To be used with mouse-on-rat, rabbit-on-rodent and goat micro-polymer detection systems.

Rodent Block M

This blocking reagent, designed for use exclusively with mouse tissue, makes use of proprietary blocking agents to reduce and/or eliminate endogenous mouse IgG. To be used with mouse-on-mouse, rat-on-mouse, rabbit-on-rodent and goat micro-polymer detection systems.

XR Factor

This blocking reagent is a potent blocker for eliminating endogenous rat IgG in rat tissues. The combination of Rodent Block R and XR Factor allows primary goat, rabbit and mouse antibodies to be used on rat tissues. XR Factor is formulated for use with mouse, rabbit, or goat micro-polymer detection systems, but should not be used with the rat micro-polymer detection system.

XM Factor

This blocking reagent is a potent blocker for eliminating endogenous mouse IgG in mouse tissues. The combination of Rodent Block M and XM Factor allows primary goat, rabbit and mouse antibodies to be used on mouse tissues. XM Factor is formulated for use with rat, rabbit or goat micro-polymer detection reagents, but should not be used with any mouse micro-polymer detection systems.

Technical Notes:

Biocare’s Rodent Decloaker, Rodent Block M and R, XR Factor and XM Factor can also be used with Biocare’s Fluorescent DyLight kits. Biocare DyLight fluorescent detection reagents may be used on mouse and rat tissues by adding 1-2 drops the XR or XM Factor to the fluorescent-secondary detection reagent. Use Rodent Block M and R prior to application of the primary antibody for optimal results.

Reduced background staining with use of Rodent Block and XR Factor

Arrows point out endogenous background due to staining of IgG.



Mouse liver without Rodent Block and XR Factor

Mouse liver without Rodent Block and XR Factor



Mouse liver with Rodent Block and XR Factor

Mouse liver with Rodent Block and XR Factor


Ordering Information

Rodent Block R

$79.00$632.00 Select options

Rodent Block M

$77.00$4,689.00 Select options

XR Factor

$49.00$166.00 Select options

XM Factor

$49.00$166.00 Select options

Rodent Decloaker, 10x

$124.00$368.00 Select options

Canine Micro-polymer Detection



The Mouse-on-Canine and Rabbit-on-Canine detection polymers are specially designed for use on canine and feline tissues. These additions to Biocare’s PromARK series expand IHC detection applications to a broader range of animal tissues, resulting in increased research capability. With advanced polymer technology and a proprietary potent blocker, the detection polymers provide increased sensitivity, reduced IHC steps (no Avidin/Biotin block or Link/Probe), and virtually eliminate cross-reactivity to endogenous canine and feline IgG. These kits can be used with paraffin-embedded tissues, floating sections and frozen sections. Canine detection is suitable for both manual and automated systems such as the intelliPATH™.



Dog colon stained with CK8/18 antibody

Dog colon stained with CK8/18 antibody

Canine Micro-polymer Detection

Mouse-on-Canine HRP-Polymer

$99.00$701.00 Select options

Mouse-on-Canine AP-Polymer

$112.00$759.00 Select options

Rabbit-on-Canine HRP-Polymer

$99.00$701.00 Select options

Rabbit-on-Canine AP-Polymer

$112.00$759.00 Select options

IHC Detection of Prion Infection in Animal Tissue

Prions, or more specifically- plaques of the PrPres protein, are the causative agents of Transmissible Spongiform Encephalopathies (TSE). The manifestation of prion infection in sheep is Scrapie. Scrapie is a fatal neurodegenerative disease that can have significant impact on the economic and physical viability of a sheep flock. In moose, elk, and deer, TSE is evidenced by Chronic Wasting Disease (CWD). When a TSE infection is suspected in a sick or deceased animal, prion detection by IHC is the “gold standard” method for verifying that the illness was Scrapie or CWD.
The Prion IHC Assay is part of a complete package of product offerings based on our established protocol for Scrapie and CWD detection.

Key components of this package include:

  • Decloaking Chamber™ 2002 or 2008 system
  • Diva Decloaker
  • Biocare’s Prion IHC Assay (2 components: Kit A & Kit B)
  • intelliPATH™ FLX system

Biocare’s Prion IHC Assay features a streamlined kit format that contains blocking reagents, ready-to-use antibody, micro-polymer detection reagents and Warp Red chromogen. The Prion IHC assay is fully automated; all are reagents pre-packaged in intelliPATH vials for added simplicity and ease of use.



Sheep Spleen stained with Prion IHC Assay

Sheep Spleen stained with Prion IHC Assay


Sheep Brain stained with Prion IHC Assay

Sheep Brain stained with Prion IHC Assay


Ordering Information

Prion IHC Assay Kit A

$342.00 Add to cart

Prion IHC Assay Kit B

$41.00 Add to cart

anti-Prion Protein MAb F99

$394.00 Add to cart

Farma Tissues

The Mouse-on-Farma and Rabbit-on-Farma animal IHC detection polymers bring high staining sensitivity and specificity to bovine, equine, porcine and ovine tissues. Combine together for universal and simultaneous double stains, enabling multiple antibodies in unique colors on a single slide. This can be completed in approximately the same time as a single antibody stain.



Muscle Specific Antigen on Equine Artery

Muscle Specific Antigen on Equine Artery


Ordering Information

Mouse-on-Farma HRP-Polymer

$99.00$258.00 Select options

Mouse-on-Farma AP-Polymer

$107.00$303.00 Select options

Rabbit-on-Farma HRP-Polymer

$99.00$258.00 Select options

Rabbit-on-Farma AP-Polymer

$107.00$289.00 Select options

Mouse-&-Rabbit-on-Rodent Double Stain


This multiplex polymer detection is specifically designed for use on rat and mouse tissue. The 5-step procedure for detecting multiple antigens on a single slide can be done in approximately two hours using a simultaneous protocol. Staining time is reduced from 4-6 hours to approximately 2 hours by eliminating several steps: avidin-biotin blocking steps, elution step and sequential staining steps. The advanced micro-polymer technology provides simplified procedures, increased sensitivity and virtually eliminates background staining. Potent blockers such as Rodent Block M or Rodent Block R eliminate endogenous mouse or rat IgG background for the most difficult of tissues including: lymph, spleen, lung, kidney and liver.



Rat and Mouse Double Stain Polymer: Mouse Neurofilament and rabbit GFAP on rat brain

Rat & Mouse Double Stain: Mouse Neurofilament & rabbit GFAP on rat brain


Ordering Information

Mouse-&-Rabbit-on-Rodent Double Stain Polymer

$905.00 Add to cart

Canine & Feline Tissues

Universal (Mouse + Rabbit) HRP Solution

The Mouse-on-Canine HRP-Polymer may be combined in equal volumes with the Rabbit-on-Canine HRP-Polymer to prepare a universal HRP-Polymer detection that will label both mouse and rabbit antibodies with HRP.

Mouse Antibody (HRP) + Rabbit Antibody (AP) Solution

The Mouse-on-Canine HRP-Polymer may be combined in equal volumes with the Rabbit-on-Canine AP-Polymer to prepare a double stain polymer detection that will label the mouse antibody with HRP and the rabbit antibody with AP.

Rabbit Antibody (HRP) + Mouse Antibody (AP) Solution

The Rabbit-on-Canine HRP-Polymer may be combined in equal volumes with the Mouse-on-Canine AP-Polymer to prepare a double stain polymer detection that will label the rabbit antibody with HRP and the mouse antibody with AP.

Ordering Information

Mouse-on-Canine HRP-Polymer

$99.00$701.00 Select options

Rabbit-on-Canine HRP-Polymer

$99.00$701.00 Select options

Rabbit-on-Canine HRP-Polymer

$112.00$759.00 Select options

Rabbit-on-Canine AP-Polymer

$112.00$759.00 Select options

Farma Tissues

Universal (Mouse + Rabbit) HRP Solution

The Mouse-on-Farma HRP-Polymer may be combined in equal volumes with the Rabbit-on-Farma HRP-Polymer to prepare a universal HRP-Polymer detection that will label both mouse and rabbit antibodies with HRP.

Mouse Antibody (HRP) + Rabbit Antibody (AP) Solution

The Mouse-on-Farma HRP-Polymer may be combined in equal volumes with the Rabbit-on-Farma AP-Polymer to prepare a double stain polymer detection that will label the mouse antibody with HRP and the rabbit antibody with AP.

Rabbit Antibody (HRP) + Mouse Antibody (AP) Solution

The Rabbit-on-Farma HRP-Polymer may be combined in equal volumes with the Mouse-on-Farma AP-Polymer to prepare a double stain polymer detection that will label the rabbit antibody with HRP and the mouse antibody with AP.

Ordering Information

Mouse-on-Farma HRP-Polymer

$99.00$258.00 Select options

Mouse-on-Farma AP-Polymer

$107.00$303.00 Select options

Rabbit-on-Farma HRP-Polymer

$99.00$258.00 Select options

Rabbit-on-Farma AP-Polymer

$107.00$289.00 Select options

Antibody Cat. No. Species Clone C* F* B* E* O*
Beta-Catenin CM/PM406 Mouse 14/Beta-Catenin Use Predilute
Calretinin CP/PP092 Rabbit Polyclonal Use Predilute
Carcinoembryonic Antigen (CEA) CP009 Rabbit Polyclonal Use Predilute
CD3 (P) CP/PP215 Rabbit Polyclonal Use Predilute Use Predilute Use Predilute
CD3 T-Cell (M) CM/PM110 Mouse PS1 Use Predilute
CD10 CM/PM129 Mouse 56C6 Use Predilute
CD21 CM/PM142 Mouse 2G9 Use Predilute
CD31 CP303 Rat Mec13.3 Use Predilute
CD56 CM/PM164 Mouse BC56C04 Use Predilute
CD79a CM/PM067 Mouse HM47/A9 Use Predilute Use Predilute Use Predilute
CD117/c-kit CME/PME296 Rabbit Y145 Use Predilute
CDX2 CM/PM226 Mouse CDX2-88 Use Predilute
Cytokeratin 5 (CK5) PME430 Rabbit EP10601Y Use Predilute Use Predilute
Cytokeratin 14 (CK14) CM185 Mouse LL002 Use Predilute
Cytokeratin 20 (CK20) CM/PM062 Mouse Ks20.8 Use Predilute
Cytokeratin HMW CM/PM127 Mouse 34βE12 Use Predilute
Cytokeratin LMW 8/18 CM/PM056 Mouse 5D3 Use Predilute Use Predilute
Desmin CM/PM036 Mouse D33 Use Predilute Use Predilute
Factor VIII CP039 Rabbit Polyclonal Use Predilute
GATA-3 CM405 Mouse L50-823 Use Predilute
Glial Fibrillary Acidic Protein CP/PP040 Rabbit Polyclonal Use Predilute Use Predilute
Glial Fibrillary Acidic Protein CM/PM065 Mouse GA-5 Use Predilute
Helicobacter pylori CP/PP120 Rabbit Polyclonal Use Predilute
Hepatocyte Specific Antigen CM/PM166 Mouse OCH1E5 Use Predilute Use Predilute
IGF-1R CP414 Mouse BC10 Use Predilute
Inhibin, Alpha CM171 Mouse BC/R1 Use Predilute
Ki-67 CRM/PRM325 Rabbit Monoclonal Use Predilute Use Predilute Use Predilute
Ki-67 CM/PM375 Mouse MM1 Use Predilute Use Predilute Use Predilute Use Predilute
Mart-1 CM/PM077 Mouse M2-7C10 + M2-9E3 Use Predilute
Microglia (Iba1) CP290 Rabbit Polyclonal Use Predilute
MLH-1 CM/PM220 Mouse G168-15 Use Predilute
MSH2 CM/PM219 Mouse FE11 Use Predilute
MSH6 CM/PM265 Mouse BC/44 Use Predilute
Mucin 5AC CP231 Mouse 45M1 Use Predilute
Muscle Specific Actin CM079 Mouse HHF35 Use Predilute Use Predilute Use Predilute
Myoglobin PP041 Rabbit Polyclonal Use Predilute
Neurofilament CM/PM066 Mouse 2F11 Use Predilute
Neuron Specific Enolase (NSE) CM/PM049 Mouse DT01 + BC100 Use Predilute Use Predilute
p63 CM/PM163 Mouse BC4A4 Use Predilute
Pan Cytokeratin [AE1/AE3] CM/PM011 Mouse AE1/AE3 Use Predilute Use Predilute
Pan Cytokeratin [Lu-5] CM/PM043 Mouse Lu-5 Use Predilute Use Predilute
Pan Cytokeratin Plus[AE1/AE3+ 8/18] CM/PM162 Mouse AE1/AE3 + 5D3 Use Predilute Use Predilute
PAX8 CP/PP379 Rabbit Polyclonal Use Predilute
PAX8 ACI438 Mouse BC12 Use Predilute Use Predilute
PCNA CM152 Mouse PC10 Use Predilute
Retinoblastoma CP211 Mouse 1F8 Use Predilute
S100 Protein CP/PP021 Rabbit Polyclonal Use Predilute Use Predilute
Secretagogin (SCGN) CP/PP389 Rabbit Polyclonal Use Predilute
Smooth Muscle Actin CM/PM001 Mouse 1A4 Use Predilute Use Predilute Use Predilute
Smooth Muscle Actin CME305 Rabbit C04018 Use Predilute Use Predilute Use Predilute Use Predilute
Smoothelin CM/PM372 Mouse R4A Use Predilute Use Predilute
Synaptophysin CM/PM371 Mouse 27G12 Use Predilute
TTF-1 CM/PM087 Mouse 8G7G3/1 Use Predilute
Villin CM/PM094 Mouse 1D2C3 Use Predilute Use Predilute
Vimentin CM/PM048 Mouse V9 Use Predilute
Vimentin CRM/PRM312 Rabbit SP20 Use Predilute

*C = Canine, F = Feline, B = Bovine, E = Equine, O = Ovine

Disclaimers

1. A blank spot indicates only that testing has not yet been done. The antibody may or may not work on that tissue type.
2. This list is being constantly updated with additional antibodies tested based on tissue availability.
3. The optimum antibody dilution and protocols for a specific application can vary due to many factors. These include, but are not limited to: tissue perfusion, fixation, incubation times, section thickness and detection used.
4. The data sheet recommendations and protocols are based on exclusive use of Biocare products. Due to the superior sensitivity of these unique reagents, the recommended incubation times and titers listed are not applicable to other detection systems, as results may vary. Ultimately, it is the responsibility of the investigator to determine optimal conditions.

Technical Notes

1. In general, using Biocare’s Reveal Decloaker and Decloaking chamber at 95ºC for 40 minutes gives excellent results in canine/feline tissues. Refer also to the individual antibody protocols as provided on their datasheet.
2. If a concentrate is used, please consult the antibody datasheet for recommended diluent ratio and time.
3. Biocare’s predilute antibodies are generally incubated for 30-60 minutes or diluted and incubated overnight.
4. Canine and farma detection may be applied on tissue for 30-60 minutes. A good initial protocol is a 60 minute primary antibody incubation followed by a TBS buffer wash and a 30 minute detection incubation.
5. If background staining is noted in tissues, Background Punisher may be applied after the primary antibody for 10 minutes. Recommended chromogen protocols are Betazoid DAB (HRP) for 5 minutes or Warp Red (AP) for 7 minutes.
6. Double Stain Protocol: A mouse polymer and a rabbit polymer may be combined and incubated for approximately 60 minutes (Mouse HRP & Rabbit AP or Rabbit HRP & Mouse AP). A Background Punisher application for 10 minutes is recommended after the primary antibody for double staining procedures.