MACH 4™
MACH 4 is a highly sensitive universal detection system. The MACH 4 system can detect either mouse or rabbit antibodies, and is a two-step universal detection method.
MACH 4 micro-polymer system incorporates two key technologies:
- Enhanced probe to increase sensitivity 10- to 20- fold
- Increased density of enzymes bound to tertiaries
MACH 4 Features
- 10 – 20 times more sensitive than conventional dextran polymer systems
- 20 – 40 times more sensitive for nuclear staining
- Suitable to detect mouse specific anti-biotin or antidigoxigenin antibody in in situ hybridization (ISH)
- Micro-polymer allows superior specificity/sensitivity, nuclear access and primary antibody dilution
- Compatible with automated immunostainers
Compare MACH 4 (left) with leading competitor (right) for ER (1D5) staining.
MACH 4’s compact structure enables 40-fold increase in primary antibody dilution.
Biocare’s MACH 4 detection consists of two reagents: the secondary, also known as the enhancing reagent, is applied between the primary antibody and micro-polymer reagent.
MACH 4 is ideal for use with antibody solutions prepared from concentrates or prediluted antibody solutions. Due to its increased sensitivity, MACH 4 enables a significantly higher dilution of concentrated antibodies compared to other polymer-based detection systems.
Ordering Information
MACH 4 is available in a universal (anti-mouse and anti-rabbit) format, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).
| MACH 4 Detection | Description | Cat. No. | Download |
|---|---|---|---|
| MACH 4 Universal HRP-Polymer | Probe, HRP Micro-polymer | M4U534 G, H, L, MM | Data Sheet |
| MACH 4 Universal AP Polymer Kit | Probe, AP Micro-polymer | M4U536 G, H, L | Data Sheet |
