Chromogens

Biocare Medical offers the largest variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a colored end-product that can be visualized under bright-field microscopy. Biocare’s chromogens generate a broad spectrum of colors, shown in the table below.

Chromogen Color Chart

Chromogens for HRP End-Product Color
Betazoid DAB (3rd generation) Brown
Cardassian DAB (2nd generation) Black Brown
DAB (1st generation) Brown
Romulin AEC Brick Red
Bajoran Purple Purple
Vina Green Green
Deep Space Black™ Black
Chromogens for AP End-Product Color
Warp Red™ Fuchsin red
Vulcan Fast Red (VFR) Fuchsin Red
Ferangi Blue Royal Blue

Chromogens Compatible with HRP

Betazoid DAB Chromogen Kit


Betazoid DAB is the third-generation DAB product developed by Biocare. It is superior to conventional DAB and Cardassian DAB in terms of stability and staining intensity. This chromogen is compatible with horseradish peroxidase (HRP), not soluble in alcohol or xylene, and can be coverslipped just like any other DAB. When in the presence of peroxidase enzyme, Betazoid produces a brown stain. Betazoid DAB may increase antibody titers by two fold; and can be used manually or on automated stainers.



Betazoid DAB Chromogen Kit

Cardassian DAB Chromogen Kit


DAB is widely used in IHC staining and immunoblotting, DAB produces a brown precipitate that is insoluble in alcohol and xylene for permanent mounting. This system consists of a three-component system of a liquid stable DAB chromogen, DAB substrate buffer and DAB Enhancer. The enhancer adds contrast and a slight increase in staining intensity.



Cardassian DAB Chromogen Kit

Romulin AEC Chromogen Kit


A permanent mounting system for HRP versus aqueous mounting, Romulin AEC produces a brick-red stain and is not soluble in alcohol, xylene or xylene substitutes. This system consists of four components: buffer, stabilizer, chromogen and hydrogen peroxide. This system is compatible with both manual and automatic coverslippers.



Romulin AEC staining colon cancer with Pan Cytokeratin AE1/ AE3

Bajoran Purple Chromogen Kit


This is a permanent chromogen substrate kit that is compatible with horseradish peroxidase (HRP). It is not soluble in alcohol or xylene, and can be coverslipped just like DAB. When in the presence of a peroxidase enzyme, Bajoran purple produces a permanent lavender-purple stain. This product comes in a four-component system consisting of a ready-to-use buffer, stabilizer, chromogen and hydrogen peroxide. Bajoran Purple may also be used in double- and triple-stain procedures, nitrocellulose blots, and can be viewed by both brightfield and darkfield microscopy.



Bajoran Purple staining Squamous Cell Carcinoma with CK5

Vina Green™ Chromogen Kit


Vina Green is a novel permanent chromogen that produces a green stain in the presence of horseradish peroxidase (HRP). The kit consists of liquid Vina Green chromogen and buffer that are stable at least 4 hours at room temperature. Vina Green is clearly distinguishable from Warp Red and DAB on a single slide, enabling high flexibility for its application in Multiplex IHC. Developed for both manual and automated platforms, Vina Green is suitable for both immunohistochemistry (IHC) and in situ hybridization (ISH) applications including HPV, CMV, EBV, p63, TTF-1, Ki-67 and other targets antigens such as blood and lymphatic vessels, and basal and myoepithelial cells.



Vina Green Chromogen Kit staining colon cancer with Ki-67

Deep Space Black™ Chromogen Kit


Deep Space Black™ is a novel permanent chromogen that produces a dark grey to black stain in the presence of horseradish peroxidase (HRP). The kit consists of liquid Deep Space Black chromogen and buffer that is stable for 8 hours at room temperature once mixed. Deep Space Black is clearly distinguishable from Warp Red™, DAB, Vina Green™ and Ferangi Blue™ on a single slide, enabling high flexibility for Multiplex IHC™ applications. Developed for both manual and automated platforms, Deep Space Black is suitable for both immunohistochemistry (IHC) and in situ hybridization (ISH) applications including HPV, CMV, EBV, Kappa, Lambda, p63, HMWCK, TTF-1, Ki-67, and other targets/antigens such as cytokeratins, melanoma, and basal and myoepithelial cells.



Ki-67 on Human Colon Cancer counter-stained with Feulgen’s

Betazoid DAB Chromogen Kit

$75.00$1,680.00 Select options

Cardassian DAB Chromogen Kit

$70.00$171.00 Select options

DAB Chromogen Kit

$99.00$196.00 Select options

intelliPATH FLX™ DAB Chromogen Kit

$243.00 Add to cart

Romulin AEC Chromogen Kit

$196.00$785.00 Select options

Bajoran Purple Chromogen Kit

$215.00 Select options

Vina Green™ Chromogen Kit

$116.00$371.00 Select options

Deep Space Black™ Chromogen Kit

$118.00$290.00 Select options

Betazoid DAB Buffer

$60.00 Select options

DAB Enhancer

$85.00 Select options

DAB Sparkle

$46.00$178.00 Select options

Chromogens Compatible with Alkaline Phosphatase (AP)

Warp Red™ Chromogen Kit


Biocare Medical’s Warp Red is a faster, sharper and more stable red chromogen. The newly formulated Warp Red is quick and sensitive, similar to DAB, providing an accelerated protocol and consistent staining quality. The sharp and intense red stain creates superior contrast for Multiplex IHC™. These advanced features of Warp Red result in a faster turnaround time, greater staining consistency and improved flexibility.



CK8/18 Using Warp Red with 7 minute incubation

Vulcan Fast Red Chromogen Kit


Vulcan Fast Red is insoluble in organic solvents and can be coverslipped with a permanent mounting media. This chromogen produces a bright fuchsin-red precipitate in the presence of alkaline phosphatase (AP) enzyme. For optimum results, use Immunocare TBS Wash Buffer. Not for use with PBS, as PBS is a competitive inhibitor of alkaline phosphatase and will dramatically reduce staining. Vulcan Fast Red can be viewed by both brightfield and darkfield microscopy.



Vulcan Fast Red Chromogen Kit staining melanoma with S100


Ferangi Blue Chromogen Kit

Ferangi Blue can be coverslipped with a non xylene- or toluene-based permanent mounting media. This chromogen produces a bright royal blue precipitate in the presence of alkaline phosphatase enzyme. For optimum results, use Immunocare TBS Wash Buffer. Not for use with PBS.



Ferangi Blue Chromogen Kit staining colon cancer with CK8/18

Warp Red Chromogen Kit

$140.00$1,500.00 Select options

Vulcan Fast Red Chromogen Kit 2

$92.00$7,722.00 Select options

Ferangi Blue Chromogen Kit 2

$93.00$303.00 Select options

Oncore Chromogens

ONCORE Chromogens produce a colored reaction product that is visible by light microscopy when applied to an enzyme-antibody conjugate as part of an IHC procedure on the ONCORE Automated Slide Stainer. ONCORE Chromogens are available for both HRP (horseradish peroxidase) and AP (alkaline phosphatase) detection systems. DAB is a widely used chromogen for HRP detection systems while Fast Red is a widely used chromogen for AP detection systems. ONCORE Chromogens are to be used in conjunction with the appropriate ONCORE Detection, available above.

ONCORE DAB Chromogen Kit

$90.00$160.00 Select options

ONCORE Fast Red Chromogen Kit

$90.00 Add to cart