Ki-67 + Caspase-3
This Multiplex IHC was designed to provide information on cell death vs. cell proliferation rates. The Ki-67 nuclear antigen is associated with cell proliferation. It is found throughout the cell cycle that includes the G1, S, G2, and M phases; but not the (GO) phase. Apoptosis is a process, in which cells activate an intrinsic suicide mechanism that destroys themselves. The proteases that mediate this execution are called capases (cysteinyl-aspartic acid proteases). Cleaved caspase-3 detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to (Asp175). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position. This antibody does not cross react with other cleaved caspases. Cleaved caspase-3 is an affinity purified rabbit polyclonal antibody. This Multiplex cocktail of Ki-67 and Caspase-3 has been optimized with Biocare’s Decloaking Chamber, Double Stain Detection 2 and chromogens.
| Ordering Information |
Catalog Number |
Volume |
| Concentrate |
N/A |
N/A |
| Predilute |
PPM 240DS |
AA |
| intelliPATH™ |
N/A |
N/A |
| VP Echelon |
N/A |
N/A |
| A / AK = 0.1 ml |
B / BK = 0.5 ml |
C / CK = 1.0 ml |
G5 = 5.0 ml |
AA = 6.0 ml |
H / G25 = 25 ml |
L = 100 ml |
