Desmoglein 3 + Napsin A Cocktail

(L) Lung SqCC and Lung adenocarcinoma (R) stained Desmoglein 3 (DAB) + Napsin A (FR)

Desmoglein 3 (DSG3) is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Studies have shown DSG3 to have 83-95% sensitivity and 100% specificity in detecting squamous cell carcinoma (SqCC) vs. lung adenocarcinoma. DSG3 is associated with shorter survival for all lung cancer patients regardless of the histologic subtype (5-year survival of 20.9% vs. 49.5%, P < .001). Patients with atypical carcinoid tumors, lacking Desmoglein 3 expression showed a 5-year survival of 0% compared with 36.8% for DSG3 positive cases (P < .001).

Napsin A is expressed in type II pneumocytes of normal lung and in adenocarcinomas of the lung and kidney. Studies have shown that Napsin A is more sensitive (80-87%) and more specific marker than TTF-1. Napsin A is 100% specific for lung adenocarcinoma vs. 100% negative in lung SqCC. Napsin A used in combination with TTF-1 provides 93% sensitivity and 100% specificity for lung adenocarcinoma, if CK5 and Desmoglein 3 are both negative in the same section.

DSG3 is a cell membrane stain that marks lung SqCC (DAB). Napsin A is a cytoplasmic/granular stain that marks lung adenocarcinomas (Fast Red). In the vast majority of lung cancers tested, only a single antibody stain will be observed. Coexpression of both antibodies may be observed in adenosquamous cell carcinomas, or in some cases residual normal lung will stain with Napsin A. Desmoglein 3 + Napsin A are very sensitive and specific markers for discriminating between lung SqCC and lung adenocarcinoma. This antibody cocktail is extremely accurate and is 100% specific. In grades 1-2, Desmoglein 3 + Napsin A provide staining sensitivity in the mid 90% range; thus the antibody cocktail of Desmoglein 3 + Napsin A is a first-line screener for discriminating between lung adenocarcinoma vs. lung SqCC.

Ordering Information Catalog Number Volume
Concentrate N/A N/A
Predilute PPM 428DS AA
intelliPATH™ N/A N/A
VP Echelon N/A N/A
A / AK = 0.1 ml B / BK = 0.5 ml C / CK = 1.0 ml G5 = 5.0 ml AA = 6.0 ml H / G25 = 25 ml L = 100 ml
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