Antigen Retrieval Solutions

HIER Buffer


These buffers unmask epitopes that have been cross-linked by formalin fixation. These solutions are specially formulated for superior pH stability at high temperatures. All Decloaker solutions incorporate Assure™ color-coded pH indicator technology, this assures by visual inspection that the solution is at the correct dilution and pH. Products can be stored at room temperature, with the exception of EDTA. All buffers are sodium azide and thimerosal free. These products are specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens. The retrieval solutions also block endogenous peroxidase and reduce nonspecific background, except Antigen Decloaker, Nuclear Decloaker and EDTA Decloaker.

  • Assure™ pH Color Indicator
  • Superior Staining
  • Non-hazardous Solutions


Ki-67 stain using Biocare’s Diva Decloaker buffer for HIER
Ki-67 stain using Biocare’s Diva Decloaker buffer for HIER

Diva Decloaker – Preferred HIER for Human Tissue

Eliminates the need for multiple buffers including citrate, EDTA or high pH Tris. Antibody titers are doubled and tripled when compared to citrate buffer. Diva Decloaker is non-toxic, non-flammable and odorless, pH 6.2.

Rodent Decloaker – Preferred HIER for Mouse and Rat Tissue

A Novel Heat-Induced Epitope Retrieval (HIER) Solution for Rodent Tissues
Used for the unmasking of epitopes cross-linked by formalin fixation and blocking endogenous mouse and rat IgG. It has been specially formulated for use on mouse and rat tissues with emphasis on rat brain. Rodent Decloaker has been formulated to work with Biocare’s Promark series of mouse, rat, goat and rabbit polymer detection kits. It is designed for research use only, and not intended for therapeutic and diagnostic purposes.

Reveal Decloaker

Reveal is a specially formulated heat-retrieval solution. It is used in conjunction with Biocare’s Decloaking Chamber Pro. Reveal is non-toxic, non-flammable and odorless. Antibody titers are increased compared to citrate buffer, pH 6.0, and background staining is reduced significantly. Reveal also blocks endogenous peroxidase and mercury crystals.

Borg Decloaker

Borg Decloaker is a Tris-Based formulation, pH 9.5, and is non-toxic, non-flammable and odorless. Tissues can be deparaffinized and then slides can be placed into the Borg Decloaker used in conjunction with Biocare’s Decloaking Chamber or other heating methods. In most cases, antibody titers will increase compared to other heat-retrieval buffers.

Bull’s Eye Decloaker, 20X

Bull’s Eye is a 20X buffer heat retrieval solution with color-coded pH indicator that allows the end-user to block for endogenous peroxidase, block for non-specific background staining and antigen retrieve tissues simultaneously. Bull’s Eye is a universal buffer and is compatible with virtually all antibodies and thus eliminates the need for EDTA or high pH retrieval buffers.

EDTA Decloaker

An EDTA buffer formulation with a pH range of 8.5 (after dilution), with preservative. This product is specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens.

Antigen Decloaker

A specially formulated citrate buffer, with preservative, designed for superior pH stability at high-temperature incubations. The pH 6.0 formulation helps prevent the loss of pH-sensitive antigens.

Nuclear Decloaker

This is a Tris buffer formulation, pH 9.5 with preservative. Specially formulated for superior pH stability at high temperature, this product will help prevent the loss of pH-sensitive antigens.

Diva Decloaker, 10X

$126.00$368.00 Select options

Diva Decloaker, RTU

$151.00$323.00 Select options

Diva Decloaker, 20X

$368.00 Add to cart

Rodent Decloaker, 10X

$124.00$368.00 Select options

Reveal Decloaker, 10X

$340.00$357.00 Select options

Borg Decloaker, RTU

$69.00$328.00 Select options

Universal Decloaker, 10X

$353.00 Add to cart

Bull’s Eye Decloaker, 20X

$616.00 Add to cart

EDTA Decloaker, 5X

$86.00$370.00 Select options

Antigen Decloaker, 10X

$320.00 Add to cart

Nuclear Decloaker, 10X

$320.00 Add to cart

Antigen Retrieval Solutions Reference Chart

Product Name Intended Purpose Composition pH Formats Usage Instructions
Diva Decloaker Heat retrieval only (Proprietary) 6.2 RTU / 10,20X Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization
Rodent Decloaker Heat retrieval only (Proprietary) 6.0 10X Diluted in DI water; non-flammable and non-toxic; no deparaffinization
Reveal Heat retrieval only Citrate-based 6.0 RTU / 10X Concentrate is diluted in DI water; non-flammable and non-toxic; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Hot Rinse
Borg Decloaker Heat retrieval only Tris-based 9.5 RTU Used after deparaffinization; non-flammable and non-toxic; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Hot Rinse
Universal Decloaker Heat retrieval only (Proprietary) 6.0 10X Diluted in DI water; non-flammable and non-toxic; no deparaffinization
Antigen Decloaker Heat retrieval only Citrate-based 6.0 10X Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization
Bull’s Eye Decloaker Heat retrieval only (Proprietary) 6.0 20X
EDTA Decloaker Heat retrieval only EDTA-based 8.5 5x Diluted in DI water; non-flammable and non-toxic; no deparaffinization
Nuclear Decloaker Heat retrieval only Tris-based 9.5 10X Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization

Reveal and Borg HIER solutions may be used in a two-step deparaffinization and antigen retrieval in combination with Biocare’s Hot Rinse product.

Hot Rinse

Clarifying reagent to be used with Reveal or Borg HIER reagents.

Aqua DePar

Aqua DePar is a water-soluble deparaffinization reagent which can be used for IHC, H&Es, and special stains. Paraffin is completely dissolved by this non-flammable and non-hazardous, safe-to-use solution. Eliminates the use of xylenes and alcohols, reducing disposal costs and toxic waste. Aqua DePar can be used with a two-step deparaffinization retrieval protocol using Reveal or Borg HIER reagents.

Hot Rinse, 25X

$55.00$188.00 Select options

Aqua DePar, 10X

$276.00 Add to cart

Aqua DePar, 10X

$276.00 Add to cart

Deparaffinization Reference Chart

Deparaffinization Intended Use pH Formats Usage
Hot Rinse, 25X Post-retrieval “clarifier” 6.0 25x Used after deparaffinizing HIER reagents (such as Borg and Reveal) to remove residual paraffin; placed in applicable retrieval device alongside HIER solution; non-flammable* and non-toxic**
Aqua DePar, 10X Deparaffinization only 6.0 10x Diluted in DI water, then heated to 65-75° C and used only for deparaffinization; non-flammable* and non-toxic**

HIER Reference Chart

HIER Intended Use Composition pH Formats Usage
Borg Decloaker*** Heat retrieval only Tris-based 9.5 RTU Used after deparaffinization; non-flammable* and non-toxic**; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Aqua DePar
Reveal Decloaker*** Heat retrieval only Citrate-based 6.2 RTU & 10X Concentrate is diluted in DI water; non-flammable* and non-toxic**; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Aqua DePar

Slide Brite™

Slide Brite is a non-hazardous alternative to xylene for the deparaffinization and clearing of tissue sections. The flash point for this reagent is above 140°F (60°C), double that of xylene. Slide Brite has been designated non-hazardous on the basis of aquatic toxicity, and requires no hood or ventilation.

Slide Brite

$86.00 Add to cart

Slide Brite Reference Chart

Slide Brite Intended Purpose Composition pH Formats Usage Instruction
Slide Brite Deparaffinization and clearing (Proprietary) 6.2 RTU Used just like xylene; non-flammable* and non-toxic**