Antibody Diluents

CD117, 1:800 in standard PBS diluent (LEFT), compared 1:800 in Biocare’s Renoir Red (RIGHT).

  • Increase titers
  • Reduce background staining
  • Protease and IgG-free
  • Ready-to-use

Revival Series of Antibody Diluents

The Revival Series of Super Antibody Diluents: DaVinci Green, Monet Blue, Renoir Red and Van Gogh Yellow are optimized formulations for improving antibody titers and are extremely stable for long-term storage. The color-coded Revival diluents were compared to the majority of PBS-based and Tris-based diluents available. In the majority of cases, titers have been improved 2 to 4 times with either DaVinci Green, Monet Blue, Renoir Red or Van Gogh Yellow diluents. Greater dilution of primary antibodies provides higher specificity, thus potentially eliminating false positives.

DaVinci Green
A pH 7.3 diluent with protein carrier and preservative. This is an excellent universal diluent.

Renoir Red
Formulated at pH 6.0, Renoir Red is specially designed to work with Tris-based antibodies, rinsing buffers and/or detection systems that use alkaline phosphatase.

Van Gogh Yellow
Formulated at pH 6.0, this diluent uses Assure Technology with pH indicator to signal when a pH change has occurred. A change in pH may reduce staining and is indicated by a pH indicator color change from yellow to green.

Monet Blue
This is a pH 7.9 diluent which also uses Assure Technology with pH indicator. As in the previous diluent, a change in pH results in a color change. A change in diluent color from pale blue to green or yellow indicates that optimal staining may be compromised.

VP Monet Blue
This diluent has been specially formulated to work with Ventana® systems.
(Ventana is a trademark of Ventana Medical Systems, Inc.)

Specialty Antibody Diluents

HPV Diluent
Specially formulated for Biocare’s Broad Spectrum HPV Cocktail primary antibody, this antibody diluent is optimized to reduce the occurrence of false negatives, high background or changes in staining performance, that are often observed with other types of diluents.

Renaissance Background Reducing
This antibody diluent is specially formulated to reduce the occurrence of nonspecific background with antibodies used in IHC techniques: (Synaptophysin, PLAP, Kappa, and Lambda). We have added potent background-reducing agents to dramatically reduce any unwanted background staining.

Antibody Diluents Description Cat. No. Download
DaVinci Green Phosphate-based universal diluent PD900 H, L, M Data Sheet
Renoir Red pH 6.0, Tris-based solution PD904 H, L, M Data Sheet
Van Gogh Yellow pH 6.0, Phosphate based solution PD902 H, L, M Data Sheet
Monet Blue pH 7.9, Tris-based solution PD901 H, L, M Data Sheet
VP Monet Blue For Ventana® Systems VPD901 H, L Data Sheet
HPV Diluent For HPV Broad Spectrum PD906 L Data Sheet
Renaissance Background Reducing For antibodies that produce nonspecific background PD905 H, L Data Sheet