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This rabbit monoclonal Vimentin recognizes a 57-60 kDa protein, which is identified as vimentin. A study has shown that it has no crossreactivity with other closely related intermediate filament proteins such as desmin and GFAP (1). Vimentin is the main intermediate filament protein in mesenchymal cells, and therefore is of value in the differential diagnosis of undifferentiated neoplasms including melanoma and sarcoma (2).
Melanoma or sarcoma
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1. Caselitz J, et al. Malignant melanomas contain only the vimentin type of intermediate filaments. Virchows Arch A Pathol Anat Histopathol. 1983; 400(1):43-51
2. Leader M, et al. Vimentin: an evaluation of its role as a tumour marker. Histopathology. 1987; 11(1):63-72.
3. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
4. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005.