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Universal Decloaker buffer solution with color-coded pH indicator is used for antigen retrieval methods. This solution allows the user to deparaffinize and pretreat tissues in two steps. It is used in conjunction with a Biocare’s Aqua DePar and an electric pressure cooker (Decloaking Chamber). Universal Decloaker is non-toxic, non-flammable and odorless. Xylene and alcohols are not needed for deparaffinization, thus saving valuable technician time and reducing costs for reagents and hazardous waste removable. Universal Decloaker also reduces nonspecific background staining, blocks endogenous peroxidase and removes mercury crystals. Antibody titers are doubled when compared to citrate buffer, pH 6.0 and background staining is reduced significantly. Universal Decloaker incorporates a color-coded, pH indicator technology. The end-user is assured, by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperature and will help prevent the possibility of losing pH sensitive antigens. Universal Decloaker is sodium azide and thimerosal free and can be stored at room temperature.