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This DNA negative control probe consists of a random set of oligonucleotide sequences with a GC content of 40-70%. It should be used to assess non-specific staining when performing in situ hybridization. No positive staining should result with the use of this probe. The in situ hybridization technique offers an important advantage over immunohistochemistry, as it virtually lacks background, and allows a clean and sharp viewing of the histological preparation.
Approximately 20 tests at 20 microliters per test
1. Autillo-Touati A, et al. HPV typing by in situ hybridization on cervical cytologic smears with ASCUS. Acta Cytol. 1998 May- Jun;42(3):631-8. 2. Wilkinson DG. In Situ Hybridization, A Practical Approach, Oxford University Press (1992) ISBN 0 19 963327 4. 3. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.” 4. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005.