Rabbit on Rodent AP-Polymer

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SKU: RMR625 Categories: ,

Rabbit-on-Rodent AP-Polymer has been developed for use with rabbit monoclonal/polyclonal antibodies on mouse and rat tissues. Rabbit primary antibodies on mouse and rat tissues are advantageous because rabbit secondary detection systems can be used with minimum cross-reactivity to endogenous mouse and rat IgG. Advantages of Biocare Medical’s polymer technology are increased sensitivity, reduction of IHC steps and elimination of endogenous biotin. It can be used with formalin-fixed paraffin-embedded tissues, floating sections, frozen sections or cell cultures. Biocare’s Rabbit-on-Rodent AP-Polymer is ideal for single staining and may also be used in double and triple staining techniques. In some tissues such as spleen, endogenous mouse or rat IgG may be difficult to eliminate. Biocare Medical has developed an antigen retrieval solution (Rodent Decloaker) that helps reduce or eliminate endogenous IgG and non-specific background staining while simultaneously, performing antigen retrieval. Temperature dependent protocols at 80ºC, 95ºC and 125ºC can be performed using Biocare’s Decloaking Chamber. Biocare Medical has additional products to help eliminate persistent rat and mouse IgG. The XM and XR Factors are two potent blockers for eliminating endogenous mouse or rat IgG. By adding 1-2 drops (1 drop equals 33µl) of the XM or XR Factor to 2.5 ml of Rabbit-on-Rodent AP-Polymer detection, endogenous mouse or rat IgG will be reduced dramatically or completely eliminated. In addition Rodent Block M (mouse tissue) and Rodent Block R (rat tissue) block for non-specific background staining and reduce staining due to endogenous mouse or rat IgG. Rodent Block M or R is applied to the tissue prior to the primary antibody for 15 to 30 minutes.

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