Lambda Light Chain [N10/2]

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This antibody recognizes lambda light chains of human immunoglobulins, which may be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas (1-5). The most common feature of these malignancies is the restricted expression of a single light chain class. The normal human kappa/lambda ratio is approximately 2:1. The presence of clear cut light chain restriction with a kappa/lambda ratio more than 10:1 is consistent with a malignant proliferation (2,6).

Intended Use

IVD

Format

Concentrate, Predilute

Volume

0.1 ml, 1.0 ml, 6.0 ml

Source

Mouse Monoclonal

Clone

N10/2

Isotype

IgG1

Antigen

Lambda immunoglobulin light chain

Localization

Cytoplasmic

Positive Control

Tonsil or bone marrow

1. Samoszuk MK, et al. Limitations of numerical ratios for defining monoclonality of immunoglobulin light chains in B-cell lymphomas. Diagn Immunol. 1985; 3(3):133-8.
2. Bray M, Alper MG. Lambda light chain predominance as a sign of emerging lymphoma. Am J Clin Pathol. 1983 Oct; 80(4):526-8.
3. Sobol RE, et al. Use of immunoglobulin light chain analysis to detect bone marrow involvement in B-cell neoplasms. Clin Immunol Immunopathol. 1982 Jul; 24(1):139 -44.
4. Falini B, et al. Double labeled-antigen method for demonstration of intracellular antigens in paraffin-embedded tissues. J Histochem Cytochem. 1982 Jan; 30(1):21-6.
5. Marshall-Taylor CE, et al. Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffinembedded tissues and a heat-induced epitope retrieval technique. Appl Immunohistochem Mol Morphol. 2002 Sep; 10(3):258-62.
6. Kremer M, et al. Immunohistochemistry in bone marrow pathology: a useful adjunct for morphologic diagnosis. Virchows Arch. 2005 Dec; 447(6):920-37.
7. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
8. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.