IgG4 (M)

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IgG4 is an immunoglobulin G subclass of antibody. Mouse monoclonal IgG4 [HP6025] is specific for the Fc region of human IgG4. IgG4 can be helpful in the diagnosis of IgG4 related systemic disease (IgG4-RSD). IgG4-RSD, also known as IgG4-related sclerosing disease, can be found in many different organs and presents itself with such symptoms as lymphoplasmacytic infiltration, mass formation, sclerosis, obliterative phlebitis and increased expression of IgG4+ plasma cells as well as a high IgG4+/IgG+ ratio, typically >30% (1-4).

IgG4 has been shown to be overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). IgG4 may be a useful differential marker in a panel with IgG (IgG4+/ IgG+ plasma cell ratio is higher in IPT) and ALK (positive in IMT) (4,5).

In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells as well as a higher ratio of IgG4+ to IgG+ plasma cells as compared to other pulmonary lymphoid proliferations. These characteristics may aid in distinguishing PNLH from low-grade B-cell lymphoma of the bronchus-associated lymphoid tissue (BALT) (6).

Source

Mouse Monoclonal

Species Reactivity

Human

Clone

HP6025

Isotype

IgG1

Antigen

Fc region of human IgG4

Localization

Cytoplasmic

Positive Control

Spleen

1. Khosroshahi A, et al. A clinical overview of IgG4-related systemic disease. Curr Opin Rheumatol. 2011 Jan; 23(1):57-66.
2. Divatia M, Kim S, Ro J. IgG4-related sclerosing disease, an emerging entity: a review of a multi-system disease. Yonsei Med J. 2012 Jan; 53(1):15-34.
3. Sato Y, et al. Clinicopathologic analysis of IgG4-related skin disease. Mod Pathol. 2013 Apr; 26(4):523-32.
4. Saab ST, et al. IgG4 plasma cells in inflammatory myofibroblastic tumor: inflammatory marker or pathogenic link? Mod Pathol. 2011 Apr; 24(4):606-12.
5. Bhagat P, et al. Pulmonary inflammatory myofibroblastic tumor and IgG4-related inflammatory pseudotumor: a diagnostic dilemma. Virchows Arch. 2013 Dec; 463 (6):743-7.
6. Guinee DG Jr, et al. Pulmonary nodular lymphoid hyperplasia (pulmonary pseudolymphoma): the significance of increased numbers of IgG4-positive plasma cells. Am J Surg Pathol. 2010 Dec; 34(12):1812-9.
7. Brenner I, et al. Primary cutaneous marginal zone lymphomas with plasmacytic differentiation show frequent IgG4 expression. Mod Pathol. 2013 Dec; 26(12):1568-76. 8. Sepehr A, et al. IgG4+ to IgG+ plasma cells ratio of ampulla can help differentiate autoimmune pancreatitis from other “mass forming” pancreatic lesions. Am J Surg Pathol. 2008 Dec; 32(12):1770-9.
9. Hamilton RG, et al. Epitope mapping of human immunoglobulin-specific murine monoclonal antibodies with domain-switched, deleted and point-mutated chimeric antibodies. J Immunol Methods. 1993 Jan 14; 158(1):107-22.
10. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
11. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.