This antibody reacts with human von Willebrand factor. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. This protein has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The Factor VIII (Von Willebrand Factor) is synthesized by endothelial cells and stored in the Weibel-Palade granules. This antibody reacts specifically with the endothelial cells of both normal and reactive, and neoplastic blood and lymphatic vessels and a finely granular cytoplasmic staining. It also reacts with endocardium, platelets, and megakaryocytes. Factor VIII has been shown most useful in marking and identifying normal endothelial cells of their corresponding neoplasms. However, because not all endothelial cells synthesize and/or store the molecule, this antibody will mark approximately 70% of tumors of vascular origin. It is therefore recommended that both CD34 and Factor VIII be used together as a panel. Staining for factor VIII related antigen has also been used to measure angiogenesis and has been shown in some studies to predict tumor recurrence.
Von Willebrand factor
Endothelial cell of blood vessels
Normal lung and angiosarcoma
1. Obermair A, Wanner C, Bilgi S, Speiser P, Kaider A, Reinthaller A, Leodolter S, Gitsch G. Tumor angiogenesis in stage IB cervical cancer: correlation of microvessel density with survival. Am J Obstet Gynecol 1998 Feb;178(2):314-319.
2. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
3. National Committee for Clinical Laboratory Standards (NCCLS). Protection of laboratory workers from infectious diseases transmitted by blood and tissue; proposed guideline. Villanova, PA 1991;7(9). Order code M29-P.