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Copy control probes are suggested for use to ensure that the chromosome of interest is actually being tagged thus enabling accurate interpretation of any change in signal from a relevant locus specific probe that may be being used in conjunction1. Copy Control 3 is often combined with PIK3CA. Studies have shown that mutations of this gene are present in a wide variety of cancers2.
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Guidance for Fluorescence in Situ Hybridization Testing in Hematologic Disorders. Daynna J. Wolff, Adam Bagg, Linda D. Cooley, Gordon W. Dewald, Betsy A. Hirsch, Peter B. Jacky, Kathleen W. Rao, P. Nagesh Rao. J Mol Diagn. 2007 Apr; 9(2): 134–143
2. High frequency of mutations of the PIK3CA gene in human cancers. Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, Gazdar A. Prostate Cancer Prostatic Dis. 2010 Dec;13(4):311-5.
3. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.