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Bull’s Eye is a heat retrieval solution that allows the end-user to block for endogenous peroxidase and block for non-specific background staining while simultaneously antigen retrieving tissues. Bull’s Eye is a universal buffer compatible with virtually allantibodies and thus eliminates the need for EDTA or high pH retrieval buffers. Bull’s Eye can be used with Biocare’s digital electric pressure cooker (Decloaking Chamber), a steamer, water bath or microwave oven. Antibody titers are doubled and tripled when compared to citrate buffer, pH 6.0. Bull’s-Eye incorporates Assure Technology™, a color-coded high temperature pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens. Bull’s Eye is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.