Molecular

A simple in situ hybridization technology using proprietary DNA probes for rapid visualization of mRNA target expression.

The RISH™ probe technology, enables extremely stable hybridization with the mRNA target, resulting in a more abundant signal and conferring highly specific staining.

RISH™ Protocol Overview

RISH Protocol Overview


The protocol has been simplified by removal of the overnight hybridization, requirement for RNase free solutions, labware and harsh stringent wash conditions resulting in a procedure that is completed in approximately 2.5 hours.


The result is clear, with virtually no background; the chromogenic signal is easily visualized under brightfield microscopy, along with the tissue morphology on a single slide.


  • Rapid – Results in approximately 2.5 hours
  • Simple Procedure – 5 step protocol is as simple as IHC
  • Sensitive – Proprietary DNA technology designed for enhanced sensitivity & specificity
  • Easy – Simultaneously evaluate gene expression and tissue morphology
  • Archivable – Chromogenic signal is stable for extended storage

Immunoglobulin Light Chains – Kappa and Lambda

  • Differentiate immunoblastic reactions related to viral infections, such as mononucleosis, from lymphoid tumors
  • Used in the study of monoclonality of lymphoid tumors, lymphoproliferative syndromes, myelomas and immunodeficiency associated lymphoproliferative syndromes
  • Diagnose mono- or polyclonality in lymphoid tumor proliferation or reactive processes

Lambda Light Chain

Staining bone marrow myeloma of the neck
Lambda Light Chain DNA Probe - Staining bone marrow myeloma of the neck

Lambda mRNA may be detected in normal and neoplastic B-cells in human lymphoid tissue. Restriction of either Kappa or Lambda mRNA denotes monoclonality of lymphoid neoplasms and is useful in distinguishing between neoplastic and reactive lymphoid proliferations.

Kappa Light Chain

Staining bone marrow plasma cell myeloma
Kappa Light Chain DNA Probe - Staining bone marrow plasma cell myeloma

Kappa mRNA may be detected in normal and neoplastic B-cells in human lymphoid tissue. Restriction of kappa mRNA denotes monoclonality of lymphoid neoplasms and is useful in distinguishing between and reactive lymphoid proliferations.

Dual Kappa / Lambda

Staining polyclonal plasma cells
Enables simultaneous evaluation of immunoglobulin light chain ratios in one tissue section


Dual Kappa / Lambda RISH Probe - Staining polyclonal plasma cells

Kappa and / or Lambda light chain mRNA may be detected in normal and neoplastic B-cells in human lymphoid tissue. Restriction of either Kappa or Lambda mRNA denotes monoclonality of lymphoid neoplasms and is useful in distinguishing between neoplastic and reactive lymphoid proliferations. The Dual Kappa / Lambda probe cocktail can simultaneously detect polyclonal plasma cell infiltrates in a single tissue section.

EBER (Epstein-Barr virus Encoded RNA)

Epstein-Barr Encoded RNA (EBER) Probe – Staining Hodgkin’s Lymphoma

Epstein-Barr Encoded RNA (EBER) Probe - Staining Hodgkin's Lymphoma

The EBER digoxigenin labeled probe is intended for the detection of EBER RNA in cells infected by the Epstein-Barr virus, both in reactive and tumoral cells. This detection is carried out by in situ hybridization in formalin-fixed paraffin–embedded (FFPE) histological sections.

  • Detects Epstein-Barr Virus in Reed-Sternberg (RS) Cells
  • Used as a tool diagnose nasopharyngeal carcinoma
  • Used as a tool diagnose infectious mononucleosis, lymphoid/non-lymphoid tumors associated with Epstein-Barr viral infection

Cytomegalovirus (CMV)

Staining CMV infected lung cells


Cytomegalovirus (CMV) Probe - Staining CMV infected lung cells

The Cytomegalovirus (CMV) digoxigenin labeled probe is intended for the detection of CMV DNA and β2.7 RNA in tissues and cells infected by the human Cytomegalovirus.

  • Detects CMV genomic DNA & mRNA in infected tissues or cells
  • CMV is a member of the human herpes virus-5 (HHV-5) group transmited in breast milk, during organ transplantation, sexual activity or blood transfusions

Ordering Information

RISH™ Epstein-Barr Encoded RNA (EBER) Probe

$299.00 Select options

RISH™ Kappa Light Chain DNA Probe

$299.00 Select options

RISH™ Lambda Light Chain DNA Probe

$299.00 Select options

RISH™ Cytomegalovirus (CMV) Probe

$299.00 Select options

RISH™ Dual Kappa/Lambda Probe

$549.00 Add to cart

RISH™ Retrieval, 10X

$343.00 Add to cart

RISH™ AP Detection Kit

$420.00 Add to cart

RISH™ HRP Detection Kit

$393.00 Add to cart

RISH™ Dual Detection Kit

$687.00 Add to cart

RISH™ HybriSlips™ 22mm x 22mm (100 coverslips)

$66.00 Add to cart

Thermal Test Strips, 30-65°C, 1 box (10 tests)

$49.00 Add to cart

Thermal Test Strips, 49-71°C, 1 box (10 tests)

$49.00 Add to cart

Thermal Test Strips, 77-120°C, 1 box (10 tests)

$60.00 Add to cart

Detection Kit Components

RISH™ Detection Volume
RISHzyme™ Buffer 6 ml
RISHzyme™ 6 ml
RISH™ Secondary Reagent 6 ml
RISH™ HRP Tertiary Reagent 6 ml
Betazoid DAB (Diaminobenzidine) Chromogen 0.25 ml
Betazoid DAB Buffer 6 ml
DAB Sparkle 6 ml

* RISH Dual Detection Kit includes Vulcan Fast Red chromogen, buffer & additional mixing vial