Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45.
MART-1 Cocktail (M2-7C10 + M2-9E3) recognizes a protein of 18kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1). MART-1 is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. This MART-1 cocktail does not stain steroid tumors unlike Melan A [103].
S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes
| Specifications | |
|---|---|
| Intended Use | IVD |
| Source | Mouse Monoclonal, Rabbit Polyclonal |
| Species Reactivity | Human, Others not tested |
| Clone | T311 + M2-7C10 + M2-9E3+N/A |
| Isotype | IgG2a + IgG2b/kappa + IgG2b/kappa + N/A |
| Antigen | Mart-1, S100 Protein, Tyrosinase |
| Cellular Localization | Cytoplasmic, Mart-1, Nuclear, S100, Tyrosinase |
| Positive Control | Melanoma |
| Normal Tissue | Nevus, Skin |
| Abnormal Tissue | Melanoma |
| Ordering Information | Catalog Number | Volume |
|---|---|---|
| Concentrate | N/A | N/A |
| Predilute | PPM 213DS | AA, H |
| intelliPATH™ | IP 213DS | G10 |
| VP Echelon | N/A | N/A |
| A / AK = 0.1 ml | B / BK = 0.5 ml | C / CK = 1.0 ml | G5 = 5.0 ml | AA = 6.0 ml | H / G25 = 25 ml | L = 100 ml |
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| Data Sheets |
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| Download Data Sheet |
| Download intelliPATH™ Data Sheet |