Uro-2™ (CK20 + p53) can be used to differentiate urothelial reactive atypia from CIS (carcinoma in situ) in bladders. In normal urothelium, the superficial umbrella cell layer shows reactivity for CK20 only; whereas, p53 nuclear staining is absent to focal. For urothelium with reactive atypia, particularly in cases with marked atypia, CK20 and p53 staining remain identical to those seen in normal urothelium. In cases of CIS, diffuse, strong cytoplasmic reactivity for CK20 and diffuse nuclear reactivity for p53 is observed throughout the urothelium. Most high-grade dysplasia stains with p53 when compared to low-grade dysplasia, which may aid the pathologist in differentiating between high and low grade dysplasia in bladder urothelium.
Optional CD44 for use with Uro-2™:
![Reactive atypia in bladder stained with CD44 [Bajoran Purple Chromogen]. Reactive atypia in bladder stained with CD44 [Bajoran Purple Chromogen].](http://biocare.net/wp-content/uploads/380-1.jpg)
Reactive atypia stained with CD44 (PM380) [purple].
| Specifications | |
|---|---|
| Intended Use | IVD |
| Source | Mouse Monoclonal, Rabbit Monoclonal |
| Species Reactivity | Human, Others not tested |
| Clone | Ks20.8, Y5 |
| Isotype | IgG2a + IgG |
| Antigen | CK20 and p53 protein |
| Cellular Localization | CK20 (Cytoplasmic): Brown, p53 (Nuclear): Red |
| Positive Control | Colon cancers, p53 positive bladder |
| Normal Tissue | N/A |
| Abnormal Tissue | Bladder, Colon |
| Ordering Information | Catalog Number | Volume |
|---|---|---|
| Concentrate | N/A | N/A |
| Predilute | API 3001DS | AA |
| intelliPATH™ | N/A | N/A |
| VP Echelon | N/A | N/A |
| A / AK = 0.1 ml | B / BK = 0.5 ml | C / CK = 1.0 ml | G5 = 5.0 ml | AA = 6.0 ml | H / G25 = 25 ml | L = 100 ml |
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| Data Sheets |
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| Download Data Sheet |